Anti-SREBP1抗体[2A4] (ab3259)
![Western blot - Anti-SREBP1 antibody [2A4] (ab3259)](https://www.abcam.cn/ps/products/3/ab3259/Images/ab3259-347647-ab3259-clone-2A4-AP4511748-western-blot-HeLa-HAP1.jpg "Western blot - Anti-SREBP1 antibody [2A4] (ab3259)")
Key features and details
- Mouse monoclonal [2A4] to SREBP1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-SREBP1抗体[2A4]
参阅全部 SREBP1 一抗 -
描述
小鼠单克隆抗体[2A4] to SREBP1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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表位
Amino acids 301 - 407. -
阳性对照
- WB: HeLa and HAP1 whole cell lysates.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
2A4 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
- Epigenetics and Nuclear Signaling
- Transcription
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (11) |
Use a concentration of 5 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 127 kDa).
By Western blot, this antibody detects bands of 125 kDa (precursor SREBP1) and 60 - 70 kDa (cleaved SREBP1). Based on in-house testing, Abcam recommends using a 5% milk block for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 5 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 127 kDa). By Western blot, this antibody detects bands of 125 kDa (precursor SREBP1) and 60 - 70 kDa (cleaved SREBP1). Based on in-house testing, Abcam recommends using a 5% milk block for this product. |
靶标
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功能
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). -
组织特异性
Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle. -
序列相似性
Belongs to the SREBP family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻译后修饰
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding. -
细胞定位
Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. - Information by UniProt
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数据库链接
- Entrez Gene: 6720 Human
- Omim: 184756 Human
- SwissProt: P36956 Human
- Unigene: 592123 Human
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别名
- ADD 1 antibody
- bHLHd1 antibody
- Class D basic helix-loop-helix protein 1 antibody
see all
图片
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Western blot - Anti-SREBP1 antibody [2A4] (ab3259)All lanes : Anti-SREBP1 antibody [2A4] (ab3259) at 5 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HAP1 whole cell lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 127 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa (possible cleavage fragment)This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was blocked for an hour using 5% milk before ab3259 and ab181602 (rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 5ug/ml concentration and 1/20000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (92)
ab3259 被引用在 92 文献中.
- Karadeniz F et al. Dracunculin Inhibits Adipogenesis in Human Bone Marrow-Derived Mesenchymal Stromal Cells by Activating AMPK and Wnt/β-Catenin Signaling. Int J Mol Sci 23:N/A (2022). PubMed: 35054838
- Wu Z et al. Energy deprivation-induced AMPK activation inhibits milk synthesis by targeting PrlR and PGC-1α. Cell Commun Signal 20:25 (2022). PubMed: 35248054
- Shibuya K et al. AAA-ATPase valosin-containing protein binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4. J Biol Chem 298:101936 (2022). PubMed: 35430252
- Tiong TY et al. Targeting the SREBP-1/Hsa-Mir-497/SCAP/FASN Oncometabolic Axis Inhibits the Cancer Stem-like and Chemoresistant Phenotype of Non-Small Cell Lung Carcinoma Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35806291
- Cao H et al. Super-enhancer-associated INSM2 regulates lipid metabolism by modulating mTOR signaling pathway in neuroblastoma. Cell Biosci 12:158 (2022). PubMed: 36114560