重组Anti-SRD5A2抗体[EPR6281(B)] (ab124877)
![Western blot - Anti-SRD5A2 antibody [EPR6281(B)] (ab124877)](https://www.abcam.cn/ps/products/124/ab124877/Images/ab124877-138809-anti-srd5a2-antibody-epr6281b-western-blot.jpg "Western blot - Anti-SRD5A2 antibody [EPR6281(B)] (ab124877)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6281(B)] to SRD5A2
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SRD5A2抗体[EPR6281(B)]
参阅全部 SRD5A2 一抗 -
描述
兔单克隆抗体[EPR6281(B)] to SRD5A2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- LnCaP, Human testis, and PC-3 cell lysates
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
解离常数（KD）
KD = 3.55 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6281(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124877于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
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功能
Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. -
组织特异性
Expressed in high levels in the prostate and many other androgen-sensitive tissues. -
疾病相关
Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]. A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum. -
序列相似性
Belongs to the steroid 5-alpha reductase family. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6716 Human
- Entrez Gene: 64677 Rat
- Omim: 607306 Human
- SwissProt: P31213 Human
- SwissProt: P31214 Rat
- Unigene: 458345 Human
- Unigene: 9938 Rat
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别名
- 3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody
- 3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody
- 5 alpha SR2 antibody
see all
图片
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Western blot - Anti-SRD5A2 antibody [EPR6281(B)] (ab124877)All lanes : Anti-SRD5A2 antibody [EPR6281(B)] (ab124877) at 1/1000 dilution
Lane 1 : LnCaP cell lysate
Lane 2 : Human testis lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa -
![OI-RD Scanning - Anti-SRD5A2 antibody [EPR6281(B)] (ab124877)](https://www.abcam.cn/ps/products/124/ab124877/Images/ab124877-138808-anti-srd5a2-antibody-epr6281b.jpg)
OI-RD Scanning - Anti-SRD5A2 antibody [EPR6281(B)] (ab124877) -
![Anti-SRD5A2 antibody [EPR6281(B)] (ab124877)](https://www.abcam.cn/ps/products/124/ab124877/Images/ab124877-3-benefits-of-recombinant-antibodies.png)
Anti-SRD5A2 antibody [EPR6281(B)] (ab124877)
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab124877 被引用在 4 文献中.
- Cai Y et al. Cell-free fat extract restores hair loss: a novel therapeutic strategy for androgenetic alopecia. Stem Cell Res Ther 14:219 (2023). PubMed: 37612726
- Choi YJ et al. Ameliorative effect of Abeliophyllum distichum Nakai on benign prostatic hyperplasia in vitro and in vivo. Nutr Res Pract 16:419-434 (2022). PubMed: 35919286
- Wan R et al. Role of human 3a-hydroxysteroid dehydrogenase isoforms (AKR1C1-AKR1C3) in the extrahepatic metabolism of the steroidal aromatase inactivator Formestane. J Steroid Biochem Mol Biol 198:105527 (2020). PubMed: 31733346
- Choi YJ et al. In Vivo Effects of Polymerized Anthocyanin from Grape Skin on Benign Prostatic Hyperplasia. Nutrients 11:N/A (2019). PubMed: 31615010