Anti-SRD5A2抗体(ab27469)
Key features and details
- Goat polyclonal to SRD5A2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-SRD5A2抗体
参阅全部 SRD5A2 一抗 -
描述
山羊多克隆抗体to SRD5A2 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide:
QVQCQQSPVLAG
, corresponding to N terminal amino acids 2-13 of Human SRD5A2 -
阳性对照
- Human testes or prostate lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27469于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa). 1 hour primary incubation is recommended for this product. |
靶标
-
功能
Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. -
组织特异性
Expressed in high levels in the prostate and many other androgen-sensitive tissues. -
疾病相关
Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]. A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum. -
序列相似性
Belongs to the steroid 5-alpha reductase family. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 6716 Human
- Omim: 607306 Human
- SwissProt: P31213 Human
- Unigene: 458345 Human
-
别名
- 3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody
- 3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody
- 5 alpha SR2 antibody
see all
图片
-
Western blot - Anti-SRD5A2 antibody (ab27469)Anti-SRD5A2 antibody (ab27469) at 0.3 µg/ml + Human testes lysate (35ug protein in RIPA buffer)
Predicted band size: 28 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab27469 被引用在 5 文献中.
- Samuel RM et al. Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men. Cell Stem Cell 27:876-889.e12 (2020). PubMed: 33232663
- Bennett GA et al. Prenatal Stress Alters Hippocampal Neuroglia and Increases Anxiety in Childhood. Dev Neurosci 37:533-45 (2015). PubMed: 26279160
- Kelleher MA et al. Changes in neuroactive steroid concentrations after preterm delivery in the Guinea pig. Reprod Sci 20:1365-75 (2013). PubMed: 23585339
- Kelleher MA et al. Sex-dependent effect of a low neurosteroid environment and intrauterine growth restriction on foetal guinea pig brain development. J Endocrinol 208:301-9 (2011). PubMed: 21149437
- Vu TT et al. Changes in human placental 5alpha-reductase isoenzyme expression with advancing gestation: effects of fetal sex and glucocorticoid exposure. Reprod Fertil Dev 21:599-607 (2009). WB ; Human . PubMed: 19383266