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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Anti-SRC3抗体[AX15.3] - ChIP Grade (ab2782)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)
  • Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)

Key features and details

  • Mouse monoclonal [AX15.3] to SRC3 - ChIP Grade
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-SRC3抗体[AX15.3] - ChIP Grade
    参阅全部 SRC3 一抗
  • 描述

    小鼠单克隆抗体[AX15.3] to SRC3 - ChIP Grade
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Fusion protein corresponding to Human SRC3 aa 605-1294.

  • 阳性对照

    • WB: MCF-7 cell lysate, IHC-P: Human pancreas tissue.
  • 常规说明

    Locus Link: 8202. Alternative names: ACTR, AIB1, RAC3, CTG26, p/CIP, CAGH16, TNRC14, TNRC16, TRAM-1. Alias: Amplified in breast cancer-1.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 克隆

    单克隆
  • 克隆编号

    AX15.3
  • 同种型

    IgG1
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Coactivators and repressors
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies
    • Cancer
    • Cancer Metabolism
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    • Metabolism
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    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
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    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2782于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
Use a concentration of 2 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
IHC-P
1/10 - 1/100.
说明
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa).
IHC-P
1/10 - 1/100.

靶标

  • 功能

    Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. Interacts with PSMB9.
  • 组织特异性

    Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
  • 序列相似性

    Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • 结构域

    Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
  • 翻译后修饰

    Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
    Methylated by CARM1.
    Phosphorylated by IKK complex. Regulated its function.
  • 细胞定位

    Cytoplasm. Nucleus. Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
  • Target information above from: UniProt accession Q9Y6Q9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 8202 Human
    • Omim: 601937 Human
    • SwissProt: Q9Y6Q9 Human
    • Unigene: 592142 Human
    • 别名

      • ACTR antibody
      • AIB 1 antibody
      • AIB-1 antibody
      • AIB1 antibody
      • Amplified in breast cancer 1 antibody
      • Amplified in breast cancer 1 protein antibody
      • bHLHe42 antibody
      • CAGH 16 antibody
      • CAGH16 antibody
      • CBP interacting protein antibody
      • CBP-interacting protein antibody
      • Class E basic helix-loop-helix protein 42 antibody
      • CTG 26 antibody
      • CTG26 antibody
      • KAT13B / AIB1 antibody
      • MGC141848 antibody
      • NCOA 3 antibody
      • NCoA-3 antibody
      • Ncoa3 antibody
      • NCOA3_HUMAN antibody
      • Nuclear receptor coactivator 3 antibody
      • pCIP antibody
      • RAC 3 antibody
      • RAC-3 antibody
      • RAC3 antibody
      • Receptor associated coactivator 3 antibody
      • Receptor-associated coactivator 3 antibody
      • SRC 3 antibody
      • SRC-3 antibody
      • SRC3 antibody
      • Steroid receptor coactivator protein 3 antibody
      • Thyroid hormone receptor activator molecule 1 antibody
      • TNRC14 antibody
      • TNRC16 antibody
      • TRAM 1 antibody
      • TRAM-1 antibody
      • TRAM1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human pancreas tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and incubated with ab2782 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting

    • Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)
      Western blot - Anti-SRC3 antibody [AX15.3] - ChIP Grade (ab2782)
      Western blot of AIB1 on MCF-7 cell extract using ab2782. Western blot of AIB1 on MCF-7 cell extract using ab2782.

    实验方案

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (5)

    发表研究结果有使用 ab2782?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2782 被引用在 5 文献中.

    • Li W  et al. Targeting the NCOA3-SP1-TERT axis for tumor growth in hepatocellular carcinoma. Cell Death Dis 11:1011 (2020). PubMed: 33239622
    • Wagner M  et al. NCOA3 is a selective co-activator of estrogen receptor a-mediated transactivation of PLAC1 in MCF-7 breast cancer cells. BMC Cancer 13:570 (2013). ChIP ; Human . PubMed: 24304549
    • Levy N  et al. Differential regulation of native estrogen receptor-regulatory elements by estradiol, tamoxifen, and raloxifene. Mol Endocrinol 22:287-303 (2008). ChIP ; Human . PubMed: 17962382
    • Levy N  et al. Multiple transcription factor elements collaborate with estrogen receptor alpha to activate an inducible estrogen response element in the NKG2E gene. Endocrinology 148:3449-58 (2007). ChIP ; Human . PubMed: 17395694
    • Cvoro A  et al. Distinct roles of unliganded and liganded estrogen receptors in transcriptional repression. Mol Cell 21:555-64 (2006). ChIP ; Human . PubMed: 16483936

    客户评价及客户问答

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    Western blot abreview for Anti-SRC3 antibody [AX15.3] - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (huh-7)
    Loading amount
    20000 cells
    Specification
    huh-7
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 16 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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