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AB10310

Anti-SRC3抗体

Anti-SRC3 antibody

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(2 Publications)

Rabbit Polyclonal SRC3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NCOA3 aa 900-950.

查看别名

AIB1, BHLHE42, RAC3, TRAM1, NCOA3, Nuclear receptor coactivator 3, NCoA-3, ACTR, Amplified in breast cancer 1 protein, CBP-interacting protein, Class E basic helix-loop-helix protein 42, Receptor-associated coactivator 3, Steroid receptor coactivator protein 3, Thyroid hormone receptor activator molecule 1, AIB-1, pCIP, bHLHe42, RAC-3, SRC-3, TRAM-1

2 Images
Immunoprecipitation - Anti-SRC3 antibody (AB10310)
  • IP

Unknown

Immunoprecipitation - Anti-SRC3 antibody (AB10310)

Detection of SCR3 by Immunoprecipitation.

Samples : Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 μg/ml extract. Detection : Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3.

All lanes:

Immunoprecipitation - Anti-SRC3 antibody (ab10310)

Predicted band size: 155 kDa

false

Western blot - Anti-SRC3 antibody (AB10310)
  • WB

Unknown

Western blot - Anti-SRC3 antibody (AB10310)

Detection of SRC3 by Western Blot.

Samples : Nuclear extract (5 μg) from HeLa cells. Primary antibody ab10310 used at 0.33 μg/ml. Detection : Alkaline phosphatase conjugated goat anti-rabbit IgG diluted 1/3000 with NBT-BCIP as substrate for 20 min.

Detection of SCR3 by Immunoprecipitation. Samples : Nuclear extract (10 mg) from HeLa cells. Antibody used at 20 µg/ml extract. Detection : Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is SRC3.

All lanes:

Western blot - Anti-SRC3 antibody (ab10310)

Predicted band size: 155 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human NCOA3 aa 900-950. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6Q9

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mL", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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产品详情

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. SRC3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
See full target information NCOA3
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in veterinary science 9:895190 PubMed35898549

2022

Chimeric RNA TNNI2-ACTA1-V1 Regulates Cell Proliferation by Regulating the Expression of NCOA3.

Applications

Unspecified application

Species

Unspecified reactive species

Dongyu Liu,Jiaxin Li,Wanjun Hao,Xu Lin,Jiqiao Xia,Jiyuan Zhu,Shuo Yang,Xiuqin Yang

Molecular endocrinology (Baltimore, Md.) 19:644-56 PubMed15550471

2004

Overexpression of an N-terminally truncated isoform of the nuclear receptor coactivator amplified in breast cancer 1 leads to altered proliferation of mammary epithelial cells in transgenic mice.

Applications

WB

Species

Mouse

Maddalena T Tilli,Ronald Reiter,Annabell S Oh,Ralf T Henke,Kevin McDonnell,G Ian Gallicano,Priscilla A Furth,Anna Tate Riegel
View all publications
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Abcam Product Promise

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详情请参阅我们的条款与条件。
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