Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Src (phospho Y419)抗体(ab47411)

Reviews (2)Q&A (4)References (8)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src (phospho Y419) antibody (ab47411)
  • Western blot - Anti-Src (phospho Y419) antibody (ab47411)

Key features and details

  • Rabbit polyclonal to Src (phospho Y419)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Src (phospho Y419) antibody [EPR6239(2)] (ab133460)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Src (phospho Y419)抗体
    参阅全部 Src 一抗

  • 描述

    兔多克隆抗体to Src (phospho Y419)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide corresponding to Human Src aa 350-450 (phospho Y419).
    Database link: P12931

  • 阳性对照

    • Human colon carcinoma tissue; COLO205 cell extracts

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab47411于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P (1)
Use at an assay dependent concentration.
说明
WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • 翻译后修饰

    Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
    S-nitrosylation is important for activation of its kinase activity.

  • 细胞定位

    Cell membrane. Mitochondrion inner membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 形式

    This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.

  • 别名

    • ASV antibody
    • Avian sarcoma virus antibody
    • AW259666 antibody
    • c SRC antibody
    • CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA antibody
    • cSrc antibody
    • EC 2.7.10.2 antibody
    • Neuronal CSRC tyrosine specific protein kinase antibody
    • Neuronal proto-oncogene tyrosine-protein kinase Src antibody
    • Neuronal SRC antibody
    • Oncogene SRC antibody
    • OTTHUMP00000174476 antibody
    • OTTHUMP00000174477 antibody
    • p60 Src antibody
    • p60-Src antibody
    • p60c-src antibody
    • p60Src antibody
    • pp60c src antibody
    • pp60c-src antibody
    • pp60csrc antibody
    • Proto oncogene tyrosine protein kinase Src antibody
    • Proto-oncogene c-Src antibody
    • Proto-oncogene tyrosine-protein kinase Src antibody
    • Protooncogene SRC antibody
    • Protooncogene SRC Rous sarcoma antibody
    • Src antibody
    • SRC Oncogene antibody
    • SRC proto oncogene non receptor tyrosine kinase antibody
    • SRC_HUMAN antibody
    • SRC1 antibody
    • Tyrosine kinase pp60c src antibody
    • Tyrosine protein kinase SRC 1 antibody
    • Tyrosine protein kinase SRC1 antibody
    • v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog antibody
    • V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian) antibody
    • v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src (phospho Y419) antibody (ab47411)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Src (phospho Y419) antibody (ab47411)
    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue in the presence (right) and absence (left) of blocking phosphopeptide, using ab47411 at 1/50 dilution.
  • Western blot - Anti-Src (phospho Y419) antibody (ab47411)
    Western blot - Anti-Src (phospho Y419) antibody (ab47411)
    All lanes : Anti-Src (phospho Y419) antibody (ab47411) at 1/500 dilution

    Lane 1 : COLO205 cell extracts with serum
    Lane 2 : COLO205 cell extracts

    Predicted band size: 60 kDa



    Western blot analysis of extracts from COLO205 cells in the presence (lane 2) and absence (lane 1) of serum, using ab47411 at 1/500 dilution.

文献 (8)

发表研究结果有使用 ab47411？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab47411 被引用在 8 文献中.

  • Ting PC  et al. Folic acid inhibits colorectal cancer cell migration. J Nutr Biochem 63:157-164 (2019). PubMed: 30393128
  • Chen J  et al. IL-6/YAP1/ß-catenin signaling is involved in intervertebral disc degeneration. J Cell Physiol 234:5964-5971 (2019). PubMed: 30511395
  • Iida M  et al. Src inhibition attenuates polyglutamine-mediated neuromuscular degeneration in spinal and bulbar muscular atrophy. Nat Commun 10:4262 (2019). PubMed: 31537808
  • Huo YN  et al. Androgen receptor activation reduces the endothelial cell proliferation through activating the cSrc/AKT/p38/ERK/NF?B-mediated pathway. J Steroid Biochem Mol Biol 194:105459 (2019). PubMed: 31470108
  • Grun D  et al. NRP-1 interacts with GIPC1 and a6/ß4-integrins to increase YAP1/?Np63a-dependent epidermal cancer stem cell survival. Oncogene 37:4711-4722 (2018). PubMed: 29755126
  • Chernova T  et al. Long-Fiber Carbon Nanotubes Replicate Asbestos-Induced Mesothelioma with Disruption of the Tumor Suppressor Gene Cdkn2a (Ink4a/Arf). Curr Biol 27:3302-3314.e6 (2017). PubMed: 29112861
  • Fisher ML  et al. Transglutaminase Interaction with a6/ß4-Integrin Stimulates YAP1-Dependent ?Np63a Stabilization and Leads to Enhanced Cancer Stem Cell Survival and Tumor Formation. Cancer Res 76:7265-7276 (2016). PubMed: 27780825
  • Donahue TR  et al. Integrative survival-based molecular profiling of human pancreatic cancer. Clin Cancer Res 18:1352-63 (2012). IHC-P ; Human . PubMed: 22261810

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Src (phospho Y418) antibody

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrat pH6
Sample
Mouse Tissue sections (kidney)
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Aug 20 2014

Western blot abreview for Anti-Src (phospho Y418) antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Kidney)
Loading amount
10 µg
Specification
Kidney
Gel Running Conditions
Reduced Denaturing (10% Gel)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 12 2013

Question

Name: Technical Service INTERLAB CO., LTD.
Product code: 47411

Inquiry: Dear technical support team: This customer has highly interested in ab47411 (Anti-Src (phospho Y418) antibody) and ab38497 (Anti-p53 (phospho S15) antibody), he would likes to order these antibodies and test in WB with mouse sample, would you please help this customer to create these discount codes, the information of this customer as follow:

Could you please help this customer to solve these problems? Thanks for your kindly help. Best regards

Read More
Answer

ab38497 : DISCOUNT CODE: 41002

ab47411: DISCOUNT CODE: 41864

Expiration date:09. October 2012

I am very pleased to hear your customer would like to accept our offer and test ab38497 and ab47411in mouse.

Eachcode will give your customer 1 freeproducts up to the value of the tested antibodybefore the expiration date.

To redeem this offer, please submit an Abreview for mouse and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests.

The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:

1) Call to place your order and mention the code to our customer service department;

2) Include the code in your fax order;

3) Place your order on the web and enter the promotional code.

Any feedback that your customer can provide will be greatly appreciated, whether positive or negative. If you or your customer has any further questions, please do not hesitate to contact us. We look forward to receiving your customer's Abreview and wish your customer luck with their research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Question

Before starting the project, can we have a small amount of
free sample to test whether it works or not ? Please let me know.

Read More
Answer

Thank you for your enquiry.

We do not stock small sample sizes for our products. Only sizes noted on the online product datasheets are available. However, it seems you are interested in using ab47411 in IHC on paraffin embedded sections of human cancer. If this is the case, please know that this application and species has already been tested successfully with ab47411 and we will guarantee the antibody to work under these circumstances.

Please see our Abpromise guarantee for more information https://www.abcam.com/abpromise.

I hope this is helpful. Please contact me again if you have any further questions.

Read More

Question

for ab47411, ab4817, ab32078: Are the blocking peptides available?

Read More
Answer

Thank you for your patience.

I just found out that, unfortunately, the blocking peptide for ab47411 is also not available for sale. I am very sorry to not be able to help you further in this regard.

Please do not hesitate to contact us if you need any more advice or information.

Read More

Question

for ab47411, ab4817, ab32078: Are the blocking peptides available?

Read More
Answer

Thank you for contacting us.

For ab4817, ab32078 the immuinogen peptide are unfortunately not available.

I have inquired about ab47411 and will let you know when I hear back form the lab.

Thank you for your patience.In the meantime, please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com