重组Anti-Src (phospho Y416)抗体[EPR20755-26] - BSA and Azide free (Detector) (ab272314)
![Sandwich ELISA - Anti-Src (phospho Y416) antibody [EPR20755-26] - BSA and Azide free (Detector) (ab272314)](https://www.abcam.cn/ps/products/272/ab272314/Images/ab272314-2-human-src-py416-antibody-pair-sandwich-elisa-standard-curve-2.jpg "Sandwich ELISA - Anti-Src (phospho Y416) antibody [EPR20755-26] - BSA and Azide free (Detector) (ab272314)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20755-26] to Src (phospho Y416) - BSA and Azide free (Detector)
- Suitable for: Sandwich ELISA
- Reacts with: Human
概述
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产品名称
Anti-Src (phospho Y416)抗体[EPR20755-26] - BSA and Azide free (Detector)
参阅全部 Src 一抗 -
描述
兔单克隆抗体[EPR20755-26] to Src (phospho Y416) - BSA and Azide free (Detector) -
宿主
Rabbit -
经测试应用
适用于: Sandwich ELISAmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab272314 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Src. The recommended pair for sandwich ELISA is:
Capture: ab272313, Human Src Capture Antibody (unconjugated)
Detector: ab272314, Human Src (pY416) Detector Antibody (unconjugated)
The reference range value is 0.313 - 20 U/ml.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20755-26 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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sELISA pair antibody
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272314于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795).
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| 说明 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795). |
靶标
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功能
Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻译后修饰
Dephosphorylated at Tyr-530 by PTPRJ (By similarity). Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. Dephosphorylated by PTPRJ at Tyr-419. Normally maintained in an inactive conformation with the SH2 domain engaged with Tyr-530, the SH3 domain engaged with the SH2-kinase linker, and Tyr-419 dephosphorylated. Dephosphorylation of Tyr-530 as a result of protein tyrosine phosphatase (PTP) action disrupts the intramolecular interaction between the SH2 domain and Tyr-530, Tyr-419 can then become autophosphorylated, resulting in SRC activation. Phosphorylation of Tyr-530 by CSK allows this interaction to reform, resulting in SRC inactivation.
S-nitrosylation is important for activation of its kinase activity. -
细胞定位
Cell membrane. Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6714 Human
- Omim: 190090 Human
- SwissProt: P00523 Human
- SwissProt: P12931 Human
- Unigene: 195659 Human
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形式
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and LYN (P07948). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
别名
- ASV antibody
- Avian sarcoma virus antibody
- AW259666 antibody
see all
图片
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Sandwich ELISA - Anti-Src (phospho Y416) antibody [EPR20755-26] - BSA and Azide free (Detector) (ab272314)Representative standard curve from corresponding SimpleStep ELISA® Kit (ab270886).
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![Anti-Src (phospho Y416) antibody [EPR20755-26] - BSA and Azide free (Detector) (ab272314)](https://www.abcam.cn/ps/products/272/ab272314/Images/ab272314-1-benefits-of-recombinant-antibodies.png)
Anti-Src (phospho Y416) antibody [EPR20755-26] - BSA and Azide free (Detector) (ab272314)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab272314 尚未被引用在任何文献中。
