Anti-SRB4抗体(ab63812)
Key features and details
- Rabbit polyclonal to SRB4
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
概述
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产品名称
Anti-SRB4抗体 -
描述
兔多克隆抗体to SRB4 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Saccharomyces cerevisiae -
免疫原
Recombinant full length protein (Saccharomyces cerevisiae), purified by chromatography.
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阳性对照
- Saccharomyces cerevisiae extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 6
Preservative: 0.1% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63812于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa).
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说明 |
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WB
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 78 kDa). |
靶标
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功能
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The Mediator complex unfolds to an extended conformation and partially surrounds RNA polymerase II, specifically interacting with the unphosphorylated form of the C-terminal domain (CTD) of RNA polymerase II. The Mediator complex dissociates from the RNA polymerase II holoenzyme and stays at the promoter when transcriptional elongation begins. -
序列相似性
Belongs to the Mediator complex subunit 17 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 856743 Saccharomyces cerevisiae
- SwissProt: P32569 Saccharomyces cerevisiae
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别名
- MED17 antibody
- MED17_YEAST antibody
- Mediator complex subunit 17 antibody
see all
图片
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All lanes : Anti-SRB4 antibody (ab63812) at 1/5000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Saccharomyces cerevisiae extract
Predicted band size: 78 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab63812 被引用在 3 文献中.
- Young CP et al. Distinct histone methylation and transcription profiles are established during the development of cellular quiescence in yeast. BMC Genomics 18:107 (2017). WB ; Saccharomyces cerevisiae . PubMed: 28122508
- Paul E et al. Genome-wide association of mediator and RNA polymerase II in wild-type and mediator mutant yeast. Mol Cell Biol 35:331-42 (2015). ChIP ; Saccharomyces cerevisiae . PubMed: 25368384
- Jeronimo C & Robert F Kin28 regulates the transient association of Mediator with core promoters. Nat Struct Mol Biol 21:449-55 (2014). IP . PubMed: 24704787