AB140623

重组Anti-SR蛋白repeat/SRSF6抗体[EPR9231]

Anti-SR protein repeat/SRSF6 antibody [EPR9231]

RabMAb
Recombinant
KO Validated
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(4 Publications)

Rabbit Recombinant Monoclonal SR protein repeat/SRSF6 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

查看别名

SFRS6, SRP55, SRSF6, Serine/arginine-rich splicing factor 6, Pre-mRNA-splicing factor SRP55

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab140623 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labelling SR protein repeat/SRSF6 with ab140623 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140623 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140623 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

Immunohistochemical analysis of paraffin embedded normal Human uterus tissue using ab140623 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

WB

Unknown

Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

All lanes:

Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 40 kDa

false

Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

WB

Lab

Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (AB140623)

Lanes 1-4 : Merged signal (red and green). Green - ab140623 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa.

ab140623 Anti-SR protein repeat/SRSF6 antibody [EPR9231] was shown to specifically react with SR protein repeat/SRSF6 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266529 (knockout cell lysate ab258210) was used. Wild-type and SR protein repeat/SRSF6 knockout samples were subjected to SDS-PAGE. ab140623 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SR protein repeat/SRSF6 antibody [EPR9231] (ab140623) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

SRSF6 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human SRSF6 (SR protein repeat) knockout HEK-293T cell line (<a href='/products/cell-lines/human-srsf6-sr-protein-repeat-knockout-hek-293t-cell-line-ab266529'>ab266529</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

K-562 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 40 kDa

Observed band size: 48 kDa

false

不同偶联物与剂型 (8)

Carrier free

Anti-SR protein repeat/SRSF6 antibody [EPR9231] - BSA and Azide free
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-SR protein repeat/SRSF6 antibody [EPR9231]
660 APC

APC Anti-SR protein repeat/SRSF6 antibody [EPR9231]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-SR protein repeat/SRSF6 antibody [EPR9231]
578 PE

PE Anti-SR protein repeat/SRSF6 antibody [EPR9231]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-SR protein repeat/SRSF6 antibody [EPR9231]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-SR protein repeat/SRSF6 antibody [EPR9231]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-SR protein repeat/SRSF6 antibody [EPR9231]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9231

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB, IHC-P

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

推荐的短期储存条件

+4°C

推荐的长期储存条件

-20°C

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in constitutive splicing and modulates the selection of alternative splice sites. Plays a role in the alternative splicing of MAPT/Tau exon 10. Binds to alternative exons of TNC pre-mRNA and promotes the expression of alternatively spliced TNC. Plays a role in wound healing and in the regulation of keratinocyte differentiation and proliferation via its role in alternative splicing.

See full target information SRSF6

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Science advances 10:eado8231 PubMed39356765

2024

SRSF6 modulates histone-chaperone HIRA splicing to orchestrate AR and E2F activity in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Antonio J Montero-Hidalgo,Juan M Jiménez-Vacas,Enrique Gómez-Gómez,Francisco Porcel-Pastrana,Prudencio Sáez-Martínez,Jesús M Pérez-Gómez,Antonio C Fuentes-Fayos,Ricardo Blázquez-Encinas,Rafael Sánchez-Sánchez,Teresa González-Serrano,Elena Castro,Pablo J López-Soto,Julia Carrasco-Valiente,André Sarmento-Cabral,Antonio J Martinez-Fuentes,Eduardo Eyras,Justo P Castaño,Adam Sharp,David Olmos,Manuel D Gahete,Raúl M Luque

Scientific reports 10:14057 PubMed32820193

2020

Silencing Srsf6 does not modulate incomplete splicing of the huntingtin gene in Huntington's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Michael A Mason,Casandra Gomez-Paredes,Kirupa Sathasivam,Andreas Neueder,Aikaterini-Smaragdi Papadopoulou,Gillian P Bates

American journal of cancer research 10:38-59 PubMed32064152

2020

miR-193a-5p promotes pancreatic cancer cell metastasis through SRSF6-mediated alternative splicing of OGDHL and ECM1.

Applications

Unspecified application

Species

Unspecified reactive species

Manman Li,Pandi Wu,Zhaocong Yang,Siwei Deng,Lingyu Ni,Yanfeng Zhang,Liang Jin,Yi Pan

Nature communications 9:3955 PubMed30262848

2018

Regulatory mechanisms of incomplete huntingtin mRNA splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Andreas Neueder,Anaelle A Dumas,Agnesska C Benjamin,Gillian P Bates

View all publications

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