AB317628

重组Anti-Squalene Epoxidase抗体[EPR29195-89]

Anti-Squalene Epoxidase antibody [EPR29195-89]

KD Validated
20ul selling size
Recombinant
RabMAb
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Rabbit Recombinant Monoclonal Squalene Epoxidase antibody. Suitable for WB and reacts with Human, Mouse samples.

查看别名

ERG1, SQLE, Squalene monooxygenase, Squalene epoxidase, SE

4 Images

Supplier Data

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (AB317628)

All lanes:

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (ab317628) at 1/1000 dilution

All lanes:

Human liver cancer tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 58 kDa,64 kDa

false

Exposure time: 6s

Supplier Data

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (AB317628)

Low expression : KARPAS-299, SU-DHL-1 (PMID : 30760928).

The molecular weight observed is consistent with what has been described in the literature (PMID : 33933449).

The identity of the bands higher than 75 kDa and lower than 45 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 59 seconds; Lanes 4-6 : 158 seconds; Lanes 7-8 : 59 seconds; Lane 9 : 125 seconds.

All lanes:

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (ab317628) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

KARPAS-299 (human t cell lymphoma cell) whole cell lysate at 20 µg

Lane 3:

SU-DHL-1 (human histiocytic lymphoblast-like cell) whole cell lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

Raji (human burkitts lymphoma b lymphocyte) whole cell lysate at 20 µg

Lane 6:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

DLD-1 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 8:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 9:

HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 53 kDa,58 kDa,64 kDa,36 kDa

false

Supplier Data

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (AB317628)

The identity of the bands between 25 kDa and 37 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (ab317628) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

F9 transfected with siRNA specifically targeting Squalene Epoxidase whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 64 kDa,36 kDa

false

Exposure time: 62s

Supplier Data

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (AB317628)

All lanes:

Western blot - Anti-Squalene Epoxidase antibody [EPR29195-89] (ab317628) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

EL4 (mouse lymphoma t lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 64 kDa

false

Exposure time: 180s

不同偶联物与剂型 (1)

Carrier free

Anti-Squalene Epoxidase antibody [EPR29195-89] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR29195-89

亚型

IgG

不含载体蛋白

反应种属

Human, Mouse

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Squalene epoxidase also called squalene monooxygenase is an essential enzyme in cholesterol biosynthesis. It converts squalene into 23-oxidosqualene through an epoxidation reaction involving the addition of an oxygen atom into the squalene molecule. The enzyme has an estimated mass of about 64 kDa and is primarily expressed in the liver where cholesterol synthesis is highly active. Its activity leads to the production of sterol intermediates which are further processed to form cholesterol and other sterols.

Biological function summary

Squalene epoxidase plays an important role in maintaining sterol homeostasis by regulating the conversion of squalene an important precursor in the cholesterol synthesis pathway. This enzyme operates as a part of the endoplasmic reticulum membrane complex where it anchors and acts on substrates to produce sterol intermediates for further enzymatic actions. Its function is tightly regulated by intracellular cholesterol levels to prevent overproduction and maintain cell membrane integrity.

Pathways

Squalene epoxidase is an important component of the mevalonate pathway a fundamental route for the synthesis of cholesterol and other isoprenoids. Besides cholesterol biosynthesis it connects to the synthesis of steroid hormones and bile acids. Farnesyl pyrophosphate another key intermediate in the mevalonate pathway influences the activity of squalene epoxidase. Moreover this enzyme cooperates with subsequent enzymes like lanosterol synthase to facilitate the continuation of sterol biosynthesis.

Dysregulation of squalene epoxidase can lead to conditions such as hypercholesterolemia which is associated with elevated cholesterol levels and cardiovascular disease. This enzyme's malfunction is also implicated in certain dermatological disorders due to disrupted sterol metabolism affecting skin barrier function. Alterations in squalene epoxidase activity can influence the expression and function of other proteins like HMG-CoA reductase which is tightly regulated by feedback mechanisms in response to intracellular cholesterol levels.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.

See full target information SQLE

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Abcam Product Promise

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详情请参阅我们的条款与条件。

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For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Squalene Epoxidase抗体[EPR29195-89]