AB211324

Anti-SQSTM1 / p62 (phospho S349) 抗体 [EPR20451]

Name: SQSTM1 / p62(磷酸化S349)抗体[EPR20451] (ab211324)| Abcam中文官网
Brand: Abcam Limited
SKU: AB211324
Price: 2734 CNY
Availability: InStock
Rating: 5 (3 reviews)

Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451]

KO Validated
RabMAb
Recombinant
Lab Essentials
了解详情

(3 Reviews)

(28 Publications)

Rabbit Recombinant Monoclonal SQSTM1 / p62 phospho S349 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 28 publications.

查看别名

ORCA, OSIL, SQSTM1, Sequestosome-1, EBI3-associated protein of 60 kDa, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBIAP, p60, p62

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (AB211324)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (AB211324)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, treated with 2μM MG-132 (ab141003) for 18 hours or untreated, labeling SQSTM1 / p62 (phospho S349) with ab211324 at 1/100 dilution, followed by Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) secondary antibody at 1/200 dilution (green).

Confocal image showing cytoplasmic staining on HeLa cell line. The expression increased after treatment with 2μM MG-132 (ab141003) for 18 hours.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (AB211324)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (AB211324)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, treated with 2μM MG-132 (ab141003) for 18 hours (red) or untreated (green), labeling SQSTM1 / p62 (phospho S349) with ab211324 at 1/500 dilution compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

Lab

Immunoprecipitation - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (AB211324)

SQSTM1 / p62 (phospho S349) was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate treated with 2μM MG-132 (ab141003) for 18h with ab211324 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab211324 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : HeLa treated with 2μM MG-132 (ab141003) for 18h whole cell lysate, 10 μg (Input).
Lane 2 : ab211324 IP in HeLa treated with 2μM MG-132 (ab141003) for 18h whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab211324 in HeLa treated with 2μM MG-132 (ab141003) for 18h whole cell lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (ab211324)

Predicted band size: 47 kDa

false

Exposure time: 10s

Collaborator

Western blot - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (AB211324)

ab211324 was shown to react with SQSTM1 in wild-type U-2 OS cells in Western blot with loss of signal observed in a SQSTM1 knockout cell line. Wild-type U-2 OS and SQSTM1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab211324 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (ab211324) at 1/1000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 20 µg

Lane 2:

SQSTM1 knockout U-2 OS cell lysate at 20 µg

Predicted band size: 47 kDa

false

Unknown

Western blot - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (AB211324)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (ab211324) at 1/1000 dilution

Lane 1:

Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

HeLa whole cell lysate treated with 2μM MG-132 (<a href='/products/biochemicals/mg-132-proteasome-inhibitor-ab141003'>ab141003</a>) for 18 hours at 10 µg

Lane 3:

HeLa whole cell lysate treated with 2μM MG-132 (<a href='/products/biochemicals/mg-132-proteasome-inhibitor-ab141003'>ab141003</a>) for 18 hours, then treated with Alkaline Phosphatase for 1 hour at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 62 kDa

false

Exposure time: 1min

Lab

Western blot - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (AB211324)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (ab211324) at 1/1000 dilution

Lane 1:

Untreated C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

C6 whole cell lysate treated with 2μM MG-132 (<a href='/products/biochemicals/mg-132-proteasome-inhibitor-ab141003'>ab141003</a>) for 18 hours at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 34 kDa,47 kDa,76 kDa

Observed band size: 42 kDa,62 kDa,76 kDa

false

Exposure time: 1min

Lab

Western blot - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (AB211324)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (ab211324) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 whole cell lysate treated with 2μM MG-132 (<a href='/products/biochemicals/mg-132-proteasome-inhibitor-ab141003'>ab141003</a>) for 18 hours at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 62 kDa

false

Exposure time: 4s

不同偶联物与剂型 (7)

565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451]
578 PE

PE Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451]
660 APC

APC Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451]
Carrier free

Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR20451

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, Flow Cyt (Intra), ICC/IF, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a bridge between polyubiquitinated proteins and autophagosomes (PubMed : 15340068, PubMed : 15953362, PubMed : 16286508, PubMed : 17580304, PubMed : 20168092, PubMed : 22017874, PubMed : 22622177, PubMed : 24128730, PubMed : 28404643, PubMed : 29343546, PubMed : 29507397, PubMed : 31857589, PubMed : 33509017, PubMed : 34471133, PubMed : 34893540, PubMed : 35831301, PubMed : 37306101, PubMed : 37802024). Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps (PubMed : 16286508, PubMed : 20168092, PubMed : 22622177, PubMed : 24128730, PubMed : 28404643, PubMed : 29343546, PubMed : 29507397, PubMed : 34893540, PubMed : 37802024). SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies (PubMed : 15911346, PubMed : 20168092, PubMed : 22017874, PubMed : 24128730, PubMed : 29343546, PubMed : 29507397, PubMed : 31857589, PubMed : 37802024). SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins (PubMed : 16286508, PubMed : 17580304, PubMed : 20168092, PubMed : 22622177, PubMed : 24128730, PubMed : 28404643, PubMed : 37802024). SQSTM1 is itself degraded along with its ubiquitinated cargos (PubMed : 16286508, PubMed : 17580304, PubMed : 37802024). Also required to recruit ubiquitinated proteins to PML bodies in the nucleus (PubMed : 20168092). Also involved in autophagy of peroxisomes (pexophagy) in response to reactive oxygen species (ROS) by acting as a bridge between ubiquitinated PEX5 receptor and autophagosomes (PubMed : 26344566). Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1 : interaction inactivates the BCR(KEAP1) complex by sequestering the complex in inclusion bodies, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes (PubMed : 20452972, PubMed : 28380357, PubMed : 33393215, PubMed : 37306101). Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes (PubMed : 29496741). Involved in endosome organization by retaining vesicles in the perinuclear cloud : following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport (PubMed : 27368102, PubMed : 33472082). Sequesters tensin TNS2 into cytoplasmic puncta, promoting TNS2 ubiquitination and proteasomal degradation (PubMed : 25101860). May regulate the activation of NFKB1 by TNF, nerve growth factor (NGF) and interleukin-1 (PubMed : 10356400, PubMed : 10747026, PubMed : 11244088, PubMed : 12471037, PubMed : 16079148, PubMed : 19931284). May play a role in titin/TTN downstream signaling in muscle cells (PubMed : 15802564). Adapter that mediates the interaction between TRAF6 and CYLD (By similarity).

See full target information SQSTM1 phospho S349

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文献 (28)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:356 PubMed40629418

2025

DPSCs modulate synovial macrophage polarization and efferocytosis via PINK1/Parkin-dependent mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjin Ma,Xinyu Wang,Dalei Sun,Jiali Chen,Linyi Zhou,Kaiao Zou,Xinxin Ni,Hongting Jin,Jun Lin

F1000Research 12:324 PubMed39006307

2024

The identification of high-performing antibodies for Sequestosome-1 for use in Western blot, immunoprecipitation and immunofluorescence.

Applications

Species

Human

Riham Ayoubi,Walaa Alshafie,Irina Shlaifer,Kathleen Southern,Peter S McPherson,Carl Laflamme

Experimental and therapeutic medicine 28:330 PubMed38979021

2024

Prevention of supercritical carbon dioxide fluid extract from Linnén on cutaneous squamous cell carcinomas progression following UV irradiation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Hong Luo,Hong-Juan Chen,Qing-Yuan Zhong,Hao-En He,Ying-Qi Huang,You-Chen Liu,Bin Lan,Yao-Qi Wen,Si-Liang Deng,Xian-Hua Du,Bao-Qin Lin,Ya-Xian Zhan

Molecular medicine (Cambridge, Mass.) 30:65 PubMed38773376

2024

A study on the mechanism of Beclin-1 m6A modification mediated by catalpol in protection against neuronal injury and autophagy following cerebral ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Kan Liu,Xinyan Yao,Jun Gao,Jinxi Wang,Jing Qi

Cell communication and signaling : CCS 22:146 PubMed38388414

2024

Regulation of NCOA4-mediated iron recycling ameliorates paraquat-induced lung injury by inhibiting ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Du,Lingyan Yu,Xinyi Yang,Fangchun Shao,Jun Xia,Weidong Jin,Yinhao Zhang,Guojie Lei,Ying Wang,Yanchun Li,Jun Zhang

PloS one 18:e0291905 PubMed37819868

2023

Noncanonical mechanism of Nrf2 activation by diacylglycerol polyethylene glycol adducts in normal human epidermal keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuro Miyoshi,Brian C Keller,Takashi Ashino,Satoshi Numazawa

Cell death discovery 9:286 PubMed37542074

2023

Cannabidiol-induced crosstalk of apoptosis and macroautophagy in colorectal cancer cells involves p53 and Hsp70.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Wang,Ali Bashiri Dezfouli,Mohammad Khosravi,Wolfgang Sievert,Stefan Stangl,Melissa Schwab,Zhiyuan Wu,Katja Steiger,Hu Ma,Gabriele Multhoff

Journal of advanced research 46:87-100 PubMed37003700

2023

Persistent activation of Nrf2 in a p62-dependent non-canonical manner aggravates lead-induced kidney injury by promoting apoptosis and inhibiting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Cai-Yu Lian,Bing-Xin Chu,Wei-Hao Xia,Zhen-Yong Wang,Rui-Feng Fan,Lin Wang

Frontiers in pharmacology 14:1092475 PubMed37033627

2023

Astragaloside IV alleviates 1-deoxysphinganine-induced mitochondrial dysfunction during the progression of chronic kidney disease through p62-Nrf2 antioxidant pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Gui,Qingfa Chen,Jiangsong Li,Ke Lu,Chen Li,Bin Xu,Yang Chen,Jingwen Men,Gerd A Kullak-Ublick,Weihua Wang,Zhibo Gai

International journal of environmental research and public health 20: PubMed36901549

2023

Increased Expression of Autophagy-Related Genes in Alzheimer's Disease-Type 2 Diabetes Mellitus Comorbidity Models in Cells.

Applications

Unspecified application

Species

Unspecified reactive species