Anti-SPTLC1抗体(ab176706)
Key features and details
- Rabbit polyclonal to SPTLC1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-SPTLC1抗体
参阅全部 SPTLC1 一抗 -
描述
兔多克隆抗体to SPTLC1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Horse, Chicken, Guinea pig, Cow, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey
-
免疫原
Synthetic peptide within Human SPTLC1 aa 50-100. The exact sequence is proprietary. NP_006406.1.
Sequence:SDLTVKEKEELIEEWQPEPLVPPVPKDHPALNYNIVSGPPSHKTVVNGKE C
Database link: O15269 -
阳性对照
- HeLa, 293T, Jurkat and NIH3T3 whole cell lysates.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176706于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 53 kDa.
|
|
| IP |
Use at 2-10 µg/mg of lysate.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 53 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
靶标
-
功能
Serine palmitoyltransferase (SPT). The heterodimer formed with LCB2 (SPTLC2 or SPTLC3) constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SSSPTA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SSSPTA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SSSPTB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SSSPTB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. -
组织特异性
Widely expressed. Not detected in small intestine. -
通路
Lipid metabolism; sphingolipid metabolism. -
疾病相关
Defects in SPTLC1 are the cause of hereditary sensory and autonomic neuropathy type 1A (HSAN1A) [MIM:162400]. The hereditary sensory and autonomic neuropathies are a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN1A is an autosomal dominant axonal neuropathy with onset in the second or third decades. Initial symptoms are loss of pain, touch, heat, and cold sensation over the feet, followed by distal muscle wasting and weakness. Loss of pain sensation leads to chronic skin ulcers and distal amputations. -
序列相似性
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 426145 Chicken
- Entrez Gene: 739412 Chimpanzee
- Entrez Gene: 614165 Cow
- Entrez Gene: 100726468 Guinea pig
- Entrez Gene: 10558 Human
- Entrez Gene: 268656 Mouse
- Entrez Gene: 100344536 Rabbit
- Entrez Gene: 705324 Rhesus monkey
see all -
别名
- HSAN antibody
- HSAN1 antibody
- HSN1 antibody
see all
图片
-
Western blot - Anti-SPTLC1 antibody (ab176706)All lanes : Anti-SPTLC1 antibody (ab176706) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-SPTLC1 antibody (ab176706)ab176706 at 0.4 µg/ml detecting SPTLC1 in HeLa whole cell lysate by WB following IP.
Lane 1: ab176706 at 6 µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of SPTLC1.
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds.
数据表及文件
-
Datasheet download
文献 (6)
ab176706 被引用在 6 文献中.
- Hwang S et al. Consequences of aneuploidy in human fibroblasts with trisomy 21. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33526671
- Bugajev V et al. Crosstalk between ORMDL3, serine palmitoyltransferase, and 5-lipoxygenase in the sphingolipid and eicosanoid metabolic pathways. J Lipid Res 62:100121 (2021). PubMed: 34560079
- Supruniuk E et al. Pyrroloquinoline Quinone Modifies Lipid Profile, but Not Insulin Sensitivity, of Palmitic Acid-Treated L6 Myotubes. Int J Mol Sci 21:N/A (2020). PubMed: 33171690
- Hwang S et al. Suppressing Aneuploidy-Associated Phenotypes Improves the Fitness of Trisomy 21 Cells. Cell Rep 29:2473-2488.e5 (2019). PubMed: 31747614
- Signorelli P et al. De novo ceramide synthesis is involved in acute inflammation during labor. Biol Chem 397:147-55 (2016). PubMed: 26501163
- Pesciotta EN et al. Dysferlin and other non-red cell proteins accumulate in the red cell membrane of Diamond-Blackfan Anemia patients. PLoS One 9:e85504 (2014). WB ; Human . PubMed: 24454878
