重组Anti-Sprouty 4/Spry-4抗体[EPR12127] (ab176337)
![Western blot - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337)](https://www.abcam.cn/ps/products/176/ab176337/Images/ab176337-203604-anti-sprouty-4-spry-4-antibody-epr12127-western-blot.jpg "Western blot - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12127] to Sprouty 4/Spry-4
- Suitable for: IP, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Sprouty 4/Spry-4抗体[EPR12127]
参阅全部 Sprouty 4/Spry-4 一抗 -
描述
兔单克隆抗体[EPR12127] to Sprouty 4/Spry-4 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HepG2, MCF7, HeLa, and K562 cell lysates. IP: MCF7 cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12127 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176337于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/10 - 1/100.
|
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
|
| 说明 |
|---|
|
IP
1/10 - 1/100. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
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功能
Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. -
序列相似性
Belongs to the sprouty family.
Contains 1 SPR (sprouty) domain. -
结构域
The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles. -
细胞定位
Cytoplasm. Cell projection > ruffle membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 81848 Human
- Entrez Gene: 24066 Mouse
- Entrez Gene: 291610 Rat
- Omim: 607984 Human
- SwissProt: Q9C004 Human
- SwissProt: Q9WTP2 Mouse
- Unigene: 323308 Human
- Unigene: 42038 Mouse
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别名
- Protein sprouty homolog 4 antibody
- Sprouty 4 antibody
- Sprouty homolog 4 antibody
see all
图片
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Western blot - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337)All lanes : Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa -
![Immunoprecipitation - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337)](https://www.abcam.cn/ps/products/176/ab176337/Images/ab176337-203607-anti-sprouty-4-spry-4-antibody-epr12127-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337)Western blot analysis on immunoprecipitation pellet from MCF-7 cells lysate (lane 1) or 1XPBS (negative control, lane 2) using ab176337 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337)](https://www.abcam.cn/ps/products/176/ab176337/Images/ab176337-4-benefits-of-recombinant-antibodies.png)
Anti-Sprouty 4/Spry-4 antibody [EPR12127] (ab176337)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab176337 被引用在 2 文献中.
- Pinto AT et al. SPRY4 as a Potential Mediator of the Anti-Tumoral Role of Macrophages in Anaplastic Thyroid Cancer Cells. Cancers (Basel) 15:N/A (2023). PubMed: 37686663
- Tian Y et al. MicroRNA-181 serves an oncogenic role in breast cancer via the inhibition of SPRY4. Mol Med Rep 18:5603-5613 (2018). PubMed: 30365052