AB7513

Anti-Sprouty 4/Spry-4抗体

Name: Sprouty 4/Spry-4抗体 (ab7513)| Abcam中文官网
Brand: Abcam Limited
SKU: AB7513
Price: 4489 CNY
Availability: InStock
Rating: 3 (1 reviews)

Anti-Sprouty 4/Spry-4 antibody

(1 Review)

(4 Publications)

Rabbit Polyclonal Sprouty 4/Spry-4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SPRY4 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

查看别名

Protein sprouty homolog 4, Spry-4, SPRY4

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (AB7513)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (AB7513)

ab7513 staining tumour cells in human liver tissue. Brown color indicates presence of protein, blue color shows cell nuclei.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (AB7513)

ab7513 at 2.5 μg/ml staining human heart tissue.

Unknown

Western blot - Anti-Sprouty 4/Spry-4 antibody (AB7513)

The blot was incubated with ab7516 (1/100) overnight at 4°C.

All lanes:

Western blot - Anti-Sprouty 4/Spry-4 antibody (ab7513) at 1/100 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

SW13 whole cell lysate at 30 µg

Lane 3:

C2C12 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution

Predicted band size: 33 kDa

true

Unknown

Western blot - Anti-Sprouty 4/Spry-4 antibody (AB7513)

Western blot using ab7513 at 1/500.

Lane 1 : Hela nuclear
Lane 2 : Hela
Lane 3 : A431
Lane 4 : Jurkat
Lane 5 : 293

Secondary ab : Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time : 2minutes.
Lysates at 5μg per lane.

We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD.

Western blot using ab7513 at 1/500.

Lane 1 : Hela nuclear
Lane 2 : Hela
Lane 3 : A431
Lane 4 : Jurkat
Lane 5 : 293

Secondary ab : Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time : 2minutes.
Lysates at 5μg per lane.

We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD.

All lanes:

Western blot - Anti-Sprouty 4/Spry-4 antibody (ab7513)

Predicted band size: 33 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human SPRY4 aa 250 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9C004

特异性

A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity is expected with Sprouty-4.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-10 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

This affinity purified antibody is directed against human Sprouty-4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

存储溶液

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Spry-4 protein also known as Sprouty 4 is an inhibitor of receptor tyrosine kinase signaling pathways. It weighs approximately 35 kDa and plays a role in modulating fibroblast growth factor (FGF) and epidermal growth factor (EGF) signaling. Spry-4 is expressed in various tissues including the brain lungs and kidneys. Its expression varies during different developmental stages and under different physiological conditions.

Biological function summary

Sprouty family proteins such as Spry-4 negatively regulate cell growth differentiation and angiogenesis. This protein functions as part of a negative feedback loop in growth factor-mediated signaling. By modulating signaling Spry-4 helps maintain balanced cellular responses ensuring cells do not undergo uncontrolled proliferation or differentiation. Spry-4's regulatory role is important in tissue development and repair.

Pathways

Spry-4 plays a significant role in the MAPK/ERK and PI3K/AKT pathways. In these pathways Spry-4 interacts with other proteins like RAF1 and Erk. Its inhibitory effects prevent excessive activation of these pathways which are essential for processes like cell division and survival. Spry-4's ability to modulate these pathways determines how cells respond to external stimuli maintaining cellular homeostasis.

Spry-4's dysregulation has been linked to cancer and congenital disorders. For example altered expression of Spry-4 can lead to tumorigenesis since the balance in growth factor signaling is disrupted. In cancer Spry-4 often interacts with other regulatory proteins such as SOS1 which further modulates signaling pathways important for tumor growth. In congenital disorders mutations in Spry-4 or related pathways affect normal tissue development leading to developmental anomalies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras (PubMed : 12027893). Probably impairs the formation of GTP-Ras (PubMed : 12027893). Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (PubMed : 12717443). Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed : 15584898).

See full target information SPRY4

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 58:102513 PubMed36334381

2022

Macrophage Sprouty4 deficiency diminishes sepsis-induced acute lung injury in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Chen,Chen Cao,Huimin Liu,Wanli Jiang,Rui Pan,He He,Ke Ding,Qingtao Meng

Tumour biology : the journal of the International 37:9197-207 PubMed26768617

2016

Sprouty4 mediates amphiregulin-induced down-regulation of E-cadherin and cell invasion in human ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wai-Kin So,Jung-Chien Cheng,Yingtao Liu,Congjian Xu,Jianfang Zhao,Vincent T W Chang,Peter C K Leung

Oncogene 29:4800-13 PubMed20543868

2010

SPROUTY-2 and E-cadherin regulate reciprocally and dictate colon cancer cell tumourigenicity.

Applications

Species

Human

A Barbáchano,P Ordóñez-Morán,J M García,A Sánchez,F Pereira,M J Larriba,N Martínez,J Hernández,S Landolfi,F Bonilla,H G Pálmer,J M Rojas,A Muñoz

Physiological genomics 28:193-202 PubMed16985006

2006

Microarray interrogation of human metanephric mesenchymal cells highlights potentially important molecules in vivo.

Applications

IHC-P

Species

Human

Karen L Price,David A Long,Nipurna Jina,Helen Liapis,Mike Hubank,Adrian S Woolf,Paul J D Winyard

View all publications

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Anti-Sprouty 4/Spry-4抗体

Anti-Sprouty 4/Spry-4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (AB7513)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (AB7513)

Western blot - Anti-Sprouty 4/Spry-4 antibody (AB7513)

Western blot - Anti-Sprouty 4/Spry-4 antibody (AB7513)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性