Anti-SPOCD1抗体(ab122188)
Key features and details
- Rabbit polyclonal to SPOCD1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SPOCD1抗体 -
描述
兔多克隆抗体to SPOCD1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence, corresponding to amino acids 995-1085 (SPLLSPGLEV THSSLLLAVL LPKEGLPDTA GSSPWLGKVQ KMVSFNSKVE KRYYQPDDRR PNVPLKGTPP PGGAWQQSQG RGSIAPRGIS A) of Human SPOCD1.
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阳性对照
- IHC-P: Human testis tissue; WB: U-251 MG and Human Plasma lysates; ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab122188于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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序列相似性
Contains 1 SPOC domain.
Contains 1 TFIIS central domain. - Information by UniProt
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数据库链接
- Entrez Gene: 90853 Human
- SwissProt: Q6ZMY3 Human
- Unigene: 62604 Human
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别名
- FLJ25348 antibody
- RP11-84A19.1 antibody
- SPOC domain-containing protein 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (ab122188)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-SPOCD1 antibody (ab122188)Immunocytochemistry/immunofluorescence analysis of A431 cells labelling SPOCD1 with ab122188 shows positive staining in the nucleus and nucleoli. -
Western blot - Anti-SPOCD1 antibody (ab122188)All lanes : Anti-SPOCD1 antibody (ab122188) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma (IgG/HSA depleted) lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (ab122188)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (ab122188)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPOCD1 antibody (ab122188)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab122188 被引用在 2 文献中.
- Liang J et al. SPOCD1 promotes cell proliferation and inhibits cell apoptosis in human osteosarcoma. Mol Med Rep 17:3218-3225 (2018). PubMed: 29257309
- Wang Y et al. Differential expression profile analysis of lncRNA UCA1α regulated mRNAs in bladder cancer. J Cell Biochem 119:1841-1854 (2018). PubMed: 28815726