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Anti-SPNS2抗体(ab82629)

Submit a review Q&A (2)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPNS2 antibody (ab82629)

    Key features and details

    • Rabbit polyclonal to SPNS2
    • Suitable for: IHC-P
    • Reacts with: Mouse, Rat, Human, Drosophila melanogaster, Monkey
    • Isotype: IgG

    概述

    • 产品名称

      Anti-SPNS2抗体
      参阅全部 SPNS2 一抗

    • 描述

      兔多克隆抗体to SPNS2

    • 宿主

      Rabbit

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Mouse, Rat, Human, Drosophila melanogaster, Monkey

    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • 阳性对照

      • IHC: rat ovary tissue

    • 常规说明

      Storage of very dilute antibody solutions is not recommended

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • 存储溶液

      Preservative: 0.02% Sodium azide

      Stabilization buffer: contains Phosphate buffer; stabilizing proteins; cryogenic protectant and antibody stabilizer.
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab82629于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/50 - 1/150.
    说明
    IHC-P
    1/50 - 1/150.

    靶标

    • 功能

      Sphingolipid transporter required for migration of myocardial precursors. Transports sphingosine 1-phosphate (S1P), a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. Mediates the export of S1P from cells in the extraembryonic yolk syncytial layer (YSL), thereby regulating myocardial precursor migration.

    • 序列相似性

      Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      see all

    • 别名

      • Protein spinster homolog 2 antibody
      • Spinster homolog 2 (Drosophila) antibody
      • spinster homolog 2 antibody
      • SPNS 2 antibody
      • spns2 antibody
      • SPNS2 protein antibody
      • SPNS2_HUMAN antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPNS2 antibody (ab82629)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPNS2 antibody (ab82629)

      Immunohistochemistry analysis of formalin-fixed paraffin-embedded rat ovary tissue staining SPNS2 with ab82629 at 1/50 dilution with DAB  and Hematoxylin QS counterstaining.

    文献 (2)

    发表研究结果有使用 ab82629？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab82629 被引用在 2 文献中.

    • Lin C  et al. S1P promotes corneal trigeminal neuron differentiation and corneal nerve repair via upregulating nerve growth factor expression in a mouse model. Open Life Sci 17:1324-1332 (2022). PubMed: 36313859
    • Terlizzi M  et al. Intracellular Sphingosine-1-Phosphate Receptor 3 Contributes to Lung Tumor Cell Proliferation. Cell Physiol Biochem 55:539-552 (2021). PubMed: 34473432

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Question

    Thank you for you replay and explanation. And looking forward to hearing the data for ab82629.



    Regards,

    Read More
    Answer

    Thank you for your patience.

    I can now confirm that ab82629 was tested with partly purified SPNS2 from liver tissue.

    We recommend as positive control for endogenous SPNS2 also embryonic cells or mature liver cells (FaO, HepG2).

    I hope this information is helpful and I wish a good weekend.

    Read More

    Question

    I have been using the several antibodies from your company. Two of them are quite new genes without confirmed results commercially applied in frozen sections or paraffin sections reported.




    One is Mcph1 (BRIT1, ab2612). There are some WB images, but I would like to discuss this a bit with the technique support if possible.

    Another is Spns2 (ab82629). There is no WB images and no references. I wonder if any WB test had been done by your company and I may have a look at the images.



    If I send it wrongly, sorry, I could not do it online. Please forward my questions to the corresponding team /person. Thanks!



    Kind regards,

    Read More
    Answer

    Thank you for taking the time to contact us.

    I would like to reassure you that both antibodiesare tested and covered by our guarantee for WB.

    We are happy to offer technical support. In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    I attached an image using ab82629 in WB for your information. It was done with a 1:500 dilution of the antibody with human liver samples.


    In order to discuss the protocol, I haveattached a technical questionnaire. I would appreciate if you could complete this. It will help you put the information we require together very easily.

    I would appreciate if you could also provide an image which would help us to assess the results.

    Thank you for your time and cooperation. We look forward to receiving the completed questionaire.

    Read More

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