Anti-SPNS1抗体(ab59971)
Key features and details
- Rabbit polyclonal to SPNS1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SPNS1抗体 -
描述
兔多克隆抗体to SPNS1 -
宿主
Rabbit -
特异性
This antibody reacts specifically with human 56 kDa SPNS1 protein. -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide derived from N terminal region of human SPNS1 protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59971于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (3) |
Use a concentration of 5 µg/ml.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 56 kDa.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
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WB
1/500 - 1/2000. Predicted molecular weight: 56 kDa. |
靶标
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功能
Sphingolipid transporter (By similarity). May be involved in necrotic or autophagic cell death. -
序列相似性
Belongs to the major facilitator superfamily. Spinster family. -
细胞定位
Mitochondrion inner membrane. Colocalizes with SDHB. - Information by UniProt
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数据库链接
- Entrez Gene: 83985 Human
- Entrez Gene: 73658 Mouse
- SwissProt: Q9H2V7 Human
- SwissProt: Q8R0G7 Mouse
- Unigene: 617449 Human
- Unigene: 11112 Mouse
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别名
- HSpin1 antibody
- LAT antibody
- nrs antibody
see all
图片
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Western blot - Anti-SPNS1 antibody (ab59971)All lanes : Anti-SPNS1 antibody (ab59971) at 1/1000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 49 kDa (possible isoform) -
Immunocytochemistry/ Immunofluorescence - Anti-SPNS1 antibody (ab59971)ICC/IF image of ab59971 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59971, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab59971 被引用在 1 文献中.
- Guo H et al. Multi-omics analyses reveal that HIV-1 alters CD4+ T cell immunometabolism to fuel virus replication. Nat Immunol 22:423-433 (2021). PubMed: 33767427
