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重组RabMAb

重组Anti-SPINK1/P12抗体[SP166] - BSA and Azide free (ab271943)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free (ab271943)
  • Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free (ab271943)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP166] to SPINK1/P12 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-SPINK1/P12抗体[SP166] - BSA and Azide free
    参阅全部 SPINK1/P12 一抗

  • 描述

    兔单克隆抗体[SP166] to SPINK1/P12 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length protein corresponding to Human SPINK1/P12 aa 1 to the C-terminus. Without signal peptide.
    Database link: P00995

  • 阳性对照

    • Human prostate adenocarcinoma tissue; Human pancreas lysate

  • 常规说明

    Cow: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    ab271943 is the carrier-free version of ab183347.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271943于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 8 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
WB
Use at an assay dependent concentration. Predicted molecular weight: 8 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能

    Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.
    In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.

  • 疾病相关

    Pancreatitis, hereditary
    Tropical calcific pancreatitis

  • 序列相似性

    Contains 1 Kazal-like domain.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ISK1_HUMAN antibody
      • Pancreatic secretory trypsin inhibitor antibody
      • Pancreatic serine protease inhibitor Kazal antibody
      • PCTT antibody
      • PSTI antibody
      • Serine protease inhibitor Kazal type 1 antibody
      • Serine protease inhibitor Kazal-type 1 antibody
      • Spink 3 antibody
      • SPINK1 antibody
      • Spink3 antibody
      • TaTI antibody
      • TCP antibody
      • Tumor associated trypsin inhibitor antibody
      • Tumor-associated trypsin inhibitor antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free (ab271943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free (ab271943)

      Immunohistochemical analysis of paraffin embedded Human prostate adenocarcinoma tissue labeling SPINK1/P12 with ab183347 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183347).
    • Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free (ab271943)
      Anti-SPINK1/P12 antibody [SP166] - BSA and Azide free (ab271943)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab271943？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab271943 尚未被引用在任何文献中。

    客户评价及客户问答

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    There are currently no Customer reviews or Questions for ab271943.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com