Anti-Sphingomyelin Synthase 2抗体(ab272570)
Key features and details
- Rabbit polyclonal to Sphingomyelin Synthase 2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Sphingomyelin Synthase 2抗体
参阅全部 Sphingomyelin Synthase 2 一抗 -
描述
兔多克隆抗体to Sphingomyelin Synthase 2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human Sphingomyelin Synthase 2 aa 1-100.
Database link: Q8NHU3 -
阳性对照
- WB: RT4, U-251 MG sp. IHC-P: Human lung tissue, Fallopian tube tissue, small intestine tissue, gallbladder tissue, skeletal muscle tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272570于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine (PC) and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Direction is dependent on the relative concentrations of DAG and ceramide as phosphocholine acceptors. Directly and specifically recognizes the choline head group on the substrate. Also requires two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Does not function strictly as a SM synthase. Required for cell growth. -
组织特异性
Brain, heart, kidney, liver, muscle and stomach. -
序列相似性
Belongs to the sphingomyelin synthase family. -
翻译后修饰
Palmitoylated on Cys-331, Cys-332, Cys-343 and Cys-348; which plays an important role in plasma membrane localization. -
细胞定位
Cell membrane. Golgi apparatus membrane. Predominantly plasma membrane, some in Golgi apparatus. - Information by UniProt
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数据库链接
- Entrez Gene: 166929 Human
- Omim: 611574 Human
- SwissProt: Q8NHU3 Human
- Unigene: 595423 Human
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别名
- MGC26963 antibody
- Phosphatidylcholine:ceramide cholinephosphotransferase 2 antibody
- SGMS 2 antibody
see all
图片
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Western blot - Anti-Sphingomyelin Synthase 2 antibody (ab272570)Lanes 2-3 : Anti-Sphingomyelin Synthase 2 antibody (ab272570) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : RT4
Lane 3 : U-251 MG sp -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (ab272570)Paraffin-embedded formalin-fixed Human lung tissue stained for Sphingomyelin Synthase 2 using ab272570 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (ab272570)Paraffin-embedded formalin-fixed Human Fallopian tube tissue stained for Sphingomyelin Synthase 2 using ab272570 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (ab272570)Paraffin-embedded formalin-fixed Human small intestine tissue stained for Sphingomyelin Synthase 2 using ab272570 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (ab272570)Paraffin-embedded formalin-fixed Human gallbladder tissue stained for Sphingomyelin Synthase 2 using ab272570 at 1/500 in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (ab272570)Paraffin-embedded formalin-fixed Human skeletal muscle tissue stained for Sphingomyelin Synthase 2 using ab272570 1/500 in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272570 尚未被引用在任何文献中。