AB272570

Anti-Sphingomyelin Synthase 2抗体

Name: Sphingomyelin Synthase 2抗体 (ab272570)| Abcam中文官网
Brand: Abcam Limited
SKU: AB272570
Price: 5791 CNY
Availability: InStock

Anti-Sphingomyelin Synthase 2 antibody

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Rabbit Polyclonal Sphingomyelin Synthase 2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SGMS2 aa 1-100.

查看别名

SMS2, SGMS2, Phosphatidylcholine:ceramide cholinephosphotransferase 2, Sphingomyelin synthase 2

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (AB272570)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (AB272570)

Paraffin-embedded formalin-fixed Human gallbladder tissue stained for Sphingomyelin Synthase 2 using ab272570 at 1/500 in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (AB272570)

Paraffin-embedded formalin-fixed Human Fallopian tube tissue stained for Sphingomyelin Synthase 2 using ab272570 at 1/500 in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (AB272570)

Paraffin-embedded formalin-fixed Human skeletal muscle tissue stained for Sphingomyelin Synthase 2 using ab272570 1/500 in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (AB272570)

Paraffin-embedded formalin-fixed Human small intestine tissue stained for Sphingomyelin Synthase 2 using ab272570 at 1/500 in immunohistochemical analysis.

Unknown

Western blot - Anti-Sphingomyelin Synthase 2 antibody (AB272570)

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Western blot - Anti-Sphingomyelin Synthase 2 antibody (ab272570)

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Western blot - Anti-Sphingomyelin Synthase 2 antibody (ab272570) at 0.4 µg/mL

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Molecular weight ladder

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RT4

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U-251 MG sp

Predicted band size: 42 kDa

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IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (AB272570)

Paraffin-embedded formalin-fixed Human lung tissue stained for Sphingomyelin Synthase 2 using ab272570 at 1/500 in immunohistochemical analysis.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human SGMS2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q8NHU3

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Sphingomyelin Synthase 2 also known as SMS2 is an enzyme that facilitates the conversion of ceramide and phosphatidylcholine into sphingomyelin and diacylglycerol. This reaction plays an important role in the synthesis of sphingomyelin a significant lipid component of cell membranes. SMS2 has a molecular mass of around 51 kDa. The protein exhibits expression in tissues such as the liver kidney heart and brain suggesting its importance in various physiological contexts.

Biological function summary

This enzyme contributes significantly to maintaining the structural integrity and function of cellular membranes. It is part of lipid metabolic processes and does not specifically form a known complex with other proteins; however its function impacts membrane dynamics and signal transduction. The presence of SMS2 influences the balance of ceramide and sphingomyelin affecting cellular responses to external stress factors.

Pathways

SMS2 participates in the sphingolipid metabolic pathway and the phospholipid signaling pathway. Through these pathways SMS2 is associated with regulating molecules like protein kinase C which plays a role in various cellular signaling pathways. SMS2 activity impacts lipid composition in membranes influencing downstream signaling events and other cellular processes.

An imbalance in sphingomyelin and ceramide levels can contribute to the pathogenesis of these conditions. The enzyme's activity correlates with the regulation of response protein kinase (ERK) impacting cell proliferation and survival pathways relevant to the development of these metabolic disorders. Understanding SMS2's role offers insights into therapeutic targets for related diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Sphingomyelin synthase that primarily contributes to sphingomyelin synthesis and homeostasis at the plasma membrane. Catalyzes the reversible transfer of phosphocholine moiety in sphingomyelin biosynthesis : in the forward reaction transfers phosphocholine head group of phosphatidylcholine (PC) on to ceramide (CER) to form ceramide phosphocholine (sphingomyelin, SM) and diacylglycerol (DAG) as by-product, and in the reverse reaction transfers phosphocholine from SM to DAG to form PC and CER. The direction of the reaction appears to depend on the levels of CER and DAG in the plasma membrane (PubMed : 14685263, PubMed : 17449912, PubMed : 17982138, PubMed : 18370930). Does not use free phosphorylcholine or CDP-choline as donors (PubMed : 14685263). Can also transfer phosphoethanolamine head group of phosphatidylethanolamine (PE) on to ceramide (CER) to form ceramide phosphoethanolamine (CPE) (PubMed : 19454763). Regulates receptor-mediated signal transduction via mitogenic DAG and proapoptotic CER, as well as via SM, a structural component of membrane rafts that serve as platforms for signal transduction and protein sorting (PubMed : 17449912, PubMed : 17982138). To a lesser extent, plays a role in secretory transport via regulation of DAG pool at the Golgi apparatus and its downstream effects on PRKD1 (PubMed : 18370930, PubMed : 21980337). Required for normal bone matrix mineralization (PubMed : 30779713).

See full target information SGMS2

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Anti-Sphingomyelin Synthase 2抗体

Anti-Sphingomyelin Synthase 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (AB272570)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (AB272570)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (AB272570)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (AB272570)

Western blot - Anti-Sphingomyelin Synthase 2 antibody (AB272570)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 2 antibody (AB272570)