Anti-SPF45抗体(ab114968)
Key features and details
- Rabbit polyclonal to SPF45
- Suitable for: IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-SPF45抗体
参阅全部 SPF45 一抗 -
描述
兔多克隆抗体to SPF45 -
宿主
Rabbit -
经测试应用
适用于: IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Orangutan
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免疫原
Synthetic peptide corresponding to Human SPF45 aa 25-75.
Database link: NP_116294.1 -
阳性对照
- WB: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue, mouse plasmacytoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab114968于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
IP
Use at 2-5 µg/mg of lysate. |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia. -
序列相似性
Contains 1 G-patch domain.
Contains 1 RRM (RNA recognition motif) domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 84991 Human
- Entrez Gene: 76938 Mouse
- Entrez Gene: 291295 Rat
- Omim: 606935 Human
- SwissProt: Q96I25 Human
- SwissProt: Q8JZX4 Mouse
- Unigene: 498548 Human
- Unigene: 182769 Mouse
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别名
- 45 kDa-splicing factor antibody
- RBM 17 antibody
- Rbm17 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPF45 antibody (ab114968)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling SPF45 with ab114968 at 1/1000 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-SPF45 antibody (ab114968)Detection of SPF45 by Western Blot of Immunprecipitate.
anti-SPF45 at 0.4µg/ml staining SPF45 in HeLa whole cell lysate immunoprecipitated using ab114968 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPF45 antibody (ab114968)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse plasmacytoma tissue labelling SPF45 with ab114968 at 1/500 (2µg/ml). Detection: DAB.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab114968 被引用在 1 文献中.
- Han L et al. A 5'-tRNA halve, tiRNA-Gly promotes cell proliferation and migration via binding to RBM17 and inducing alternative splicing in papillary thyroid cancer. J Exp Clin Cancer Res 40:222 (2021). PubMed: 34225773