Anti-SPATA5L1抗体(ab126354)
Key features and details
- Rabbit polyclonal to SPATA5L1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SPATA5L1抗体 -
描述
兔多克隆抗体to SPATA5L1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: TSCHCSFVSV SGADLFSPFV GDSEKVLSQI FRQARASTPA ILFLDEIDSI LGARSASKTG CDVQERVLSV LLNELDGVGL KTIERRGSKS SQQEFQEV, corresponding to amino acids 520-617 of Human SPATA5L1.
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阳性对照
- Human pancreas tissue; RT4, U251 MG and Human tonsil tissue lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126354于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 81 kDa.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 81 kDa. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
靶标
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序列相似性
Belongs to the AAA ATPase family. AFG2 subfamily. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 79029 Human
- SwissProt: Q9BVQ7 Human
- Unigene: 369657 Human
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别名
- FLJ12286 antibody
- MGC5347 antibody
- SPA5L_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-SPATA5L1 antibody (ab126354)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab126354 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Western blot - Anti-SPATA5L1 antibody (ab126354)All lanes : Anti-SPATA5L1 antibody (ab126354) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Predicted band size: 81 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPATA5L1 antibody (ab126354)ab126354 at a 1/50 dilution staining SPATA5L1 in paraffin embedded Human pancreas tissue by immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab126354 尚未被引用在任何文献中。