重组Anti-Spata18抗体[EPR13704] (ab180154)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13704] to Spata18
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Spata18抗体[EPR13704]
参阅全部 Spata18 一抗 -
描述
兔单克隆抗体[EPR13704] to Spata18 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment within Human Spata18 aa 100-250. The exact sequence is proprietary.
Database link: Q8TC71 -
阳性对照
- HepG2, HeLa, LnCaP and HT1080 cell lysates; Human kidney tissue; HepG2 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR13704 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180154于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/500.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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说明 |
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ICC/IF
1/500. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
靶标
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功能
Potential role in spermatogenesis, especially in cell differentiation from late elongate spematids to mature spermatozoa. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 132671 Human
- Entrez Gene: 73472 Mouse
- Entrez Gene: 289586 Rat
- Omim: 612814 Human
- SwissProt: Q8TC71 Human
- SwissProt: Q0P557 Mouse
- SwissProt: Q6AYL6 Rat
- Unigene: 527090 Human
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别名
- 1700067I02Rik antibody
- FLJ32906 antibody
- MGC93900 antibody
see all
图片
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All lanes : Anti-Spata18 antibody [EPR13704] (ab180154) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : HT1080 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 61 kDa -
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Spata18 with ab180154 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/250 dilution. Counter stained with Dapi.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Spata18 with ab180154 at 1/100 dilution, followed by prediluted Goat anti rabbit IgG (HRP). Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab180154 被引用在 3 文献中.
- Hamada K et al. MIEAP and ATG5 are tumor suppressors in a mouse model of BRAFV600E-positive thyroid cancer. Front Endocrinol (Lausanne) 13:932754 (2022). PubMed: 36187114
- Song WH et al. Mammalian Cell-Free System Recapitulates the Early Events of Post-Fertilization Sperm Mitophagy. Cells 10:N/A (2021). PubMed: 34572103
- Ren M et al. Extramitochondrial cardiolipin suggests a novel function of mitochondria in spermatogenesis. J Cell Biol 218:1491-1502 (2019). PubMed: 30914420