AB167190

Anti-Spartan抗体

Name: Spartan抗体 (ab167190)| Abcam中文官网
Brand: Abcam Limited
SKU: AB167190
Price: 4560 CNY
Availability: InStock

Anti-Spartan antibody

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(1 Publication)

Mouse Polyclonal Spartan antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SPRTN.

查看别名

C1orf124, DVC1, UNQ1880/PRO4323, SPRTN, DNA-dependent metalloprotease SPRTN, DNA damage protein targeting VCP, Protein with SprT-like domain at the N terminus, Spartan

1 Images

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Western blot - Anti-Spartan antibody (AB167190)

All lanes:

Western blot - Anti-Spartan antibody (ab167190) at 1 µg/mL

Lane 1:

Lysate of 293T cells transfected with Spartan at 15 µL

Lane 2:

Lysate of 293T cells (non-transfected) at 15 µL

Predicted band size: 103 kDa,40 kDa,55 kDa

false

关键信息

宿主种属

Mouse

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Recombinant Full Length Protein corresponding to Human SPRTN.

Q9H040

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Spartan also known as SPRTN (DNA Sequence Nonspecific) is a metalloprotease enzyme weighing approximately 53 kDa. It is an important player in genome maintenance and DNA repair especially in the stress response to DNA replication impediments. SPRTN is widely expressed in various tissues with particularly high expression in proliferative tissues where DNA replication is important such as the liver and bone marrow. Its main mechanical role involves recognizing and processing DNA-protein crosslinks that may hinder replication and transcription processes.

Biological function summary

SPRTN is important for DNA replication fidelity and cellular response to DNA damage. It achieves this by being part of the DNA-protein crosslink repair pathway where it cooperates with other proteins to maintain genomic stability. This function ensures replication continues correctly and prevents harmful mutations. SPRTN operates as a protease to cleave proteins trapped on DNA thereby removing obstacles and allowing replication machinery to advance. This activity demonstrates its vital role in safeguarding genetic information.

Pathways

SPRTN integrates into the DNA damage response pathways such as the Fanconi Anemia pathway coordinating with other proteins to maintain genomic integrity. Additionally SPRTN associates with the homologous recombination repair pathway where it interacts with proteins like RAD51 and BRCA1 to facilitate effective DNA repair. These pathways are essential for managing DNA damage events to minimize the risk of genomic instabilities that may lead to disorders.

SPRTN mutations have strong links to a rare genetic disorder known as Ruijs-Aalfs Syndrome characterized by progeroid features and cancer predisposition due to accumulated DNA damage. This connection highlights its role in maintaining genomic stability. Moreover deficiency in SPRTN has relevance to cancer development as its impaired function leads to compromised DNA repair allowing mutations to accumulate. The loss of this protease's activity can correlate with destabilization of genomic maintenance partners like FANCD2 potentially contributing to oncogenesis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

DNA-dependent metalloendopeptidase that mediates the proteolytic cleavage of covalent DNA-protein cross-links (DPCs) during DNA synthesis, thereby playing a key role in maintaining genomic integrity (PubMed : 27852435, PubMed : 27871365, PubMed : 27871366, PubMed : 30893605, PubMed : 32649882, PubMed : 36608669). DPCs are highly toxic DNA lesions that interfere with essential chromatin transactions, such as replication and transcription, and which are induced by reactive agents, such as UV light or formaldehyde (PubMed : 27852435, PubMed : 27871365, PubMed : 27871366, PubMed : 32649882, PubMed : 36608669). Associates with the DNA replication machinery and specifically removes DPCs during DNA synthesis (PubMed : 27852435, PubMed : 27871365, PubMed : 27871366, PubMed : 32649882). Catalyzes proteolytic cleavage of the HMCES DNA-protein cross-link following unfolding by the BRIP1/FANCJ helicase (PubMed : 36608669). Acts as a pleiotropic protease for DNA-binding proteins cross-linked with DNA, such as TOP1, TOP2A, histones H3 and H4 (PubMed : 27871366). Mediates degradation of DPCs that are not ubiquitinated, while it is not able to degrade ubiquitinated DPCs (By similarity). SPRTN activation requires polymerase collision with DPCs followed by helicase bypass of DPCs (By similarity). Involved in recruitment of VCP/p97 to sites of DNA damage (PubMed : 22902628, PubMed : 23042605, PubMed : 23042607, PubMed : 32152270). Also acts as an activator of CHEK1 during normal DNA replication by mediating proteolytic cleavage of CHEK1, thereby promoting CHEK1 removal from chromatin and subsequent activation (PubMed : 31316063). Does not activate CHEK1 in response to DNA damage (PubMed : 31316063). May also act as a 'reader' of ubiquitinated PCNA : recruited to sites of UV damage and interacts with ubiquitinated PCNA and RAD18, the E3 ubiquitin ligase that monoubiquitinates PCNA (PubMed : 22681887, PubMed : 22894931, PubMed : 22902628, PubMed : 22987070). Facilitates chromatin association of RAD18 and is required for efficient PCNA monoubiquitination, promoting a feed-forward loop to enhance PCNA ubiquitination and translesion DNA synthesis (PubMed : 22681887).

See full target information SPRTN

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 12:17491-17503 PubMed37439040

2023

FAM60A promotes osteosarcoma development and progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sun,Yu-Nan Man,Jin-Hui Cheng,Jing-Tang Li,Ya-Yun Liu

View all publications

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Anti-Spartan抗体

Anti-Spartan antibody

Western blot - Anti-Spartan antibody (AB167190)