重组Anti-SPARC抗体[EPR20121] - Low endotoxin，Azide free (ab223156)
![Flow Cytometry (Intracellular) - Anti-SPARC antibody [EPR20121] - Low endotoxin, Azide free (ab223156)](https://www.abcam.cn/ps/products/223/ab223156/Images/ab223156-2-ab207743270810207743FC.jpg "Flow Cytometry (Intracellular) - Anti-SPARC antibody [EPR20121] - Low endotoxin, Azide free (ab223156)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20121] to SPARC - Low endotoxin, Azide free
- Suitable for: Flow Cyt (Intra), WB, IP
- Knockout validated
- Reacts with: Human
概述
-
产品名称
Anti-SPARC抗体[EPR20121] - Low endotoxin，Azide free
参阅全部 SPARC 一抗 -
描述
兔单克隆抗体[EPR20121] to SPARC - Low endotoxin，Azide free -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IPmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
不与反应: Mouse -
免疫原
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: SH-SY5Y and A-375 whole cell lysates; Human bone giant cell tumor lysate; Human serum and brain lysate. Flow Cyt (intra): A-375 cells. IP: A-375 whole cell lysate.
-
常规说明
ab223156 is the carrier-free version of ab207743.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20121 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223156于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 35 kDa).
|
|
| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 35 kDa). |
|
IP
Use at an assay dependent concentration. |
靶标
-
功能
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. -
序列相似性
Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain. -
发展阶段
Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 6678 Human
- Omim: 182120 Human
- SwissProt: P09486 Human
- Unigene: 111779 Human
- Unigene: 708558 Human
-
别名
- AA517111 antibody
- Basement membrane protein 40 antibody
- Basement-membrane protein 40 antibody
see all
图片
-
Flow Cytometry (Intracellular) - Anti-SPARC antibody [EPR20121] - Low endotoxin, Azide free (ab223156)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed A-375 (Human malignant melanoma cell line) cells labeling SPARC with ab207743 at 1/70 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207743).
-
![Immunoprecipitation - Anti-SPARC antibody [EPR20121] - Low endotoxin, Azide free (ab223156)](https://www.abcam.cn/ps/products/223/ab223156/Images/ab223156-1-ab207743270809207743IP.jpg)
Immunoprecipitation - Anti-SPARC antibody [EPR20121] - Low endotoxin, Azide free (ab223156)SPARC was immunoprecipitated from 0.35 mg of A-375 (Human malignant melanoma cell line) whole cell lysate with ab207743 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab207743 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: A-375 whole cell lysate, 10µg (Input).
Lane 2: ab207743 IP in A-375 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207743 in A-375 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207743).
-
![Anti-SPARC antibody [EPR20121] - Low endotoxin, Azide free (ab223156)](https://www.abcam.cn/ps/products/223/ab223156/Images/ab223156-3-benefits-of-recombinant-antibodies.png)
Anti-SPARC antibody [EPR20121] - Low endotoxin, Azide free (ab223156)
实验方案
数据表及文件
-
Datasheet download
Certificate of Compliance
文献 (0)
ab223156 尚未被引用在任何文献中。
