Anti-SPARC抗体(ab14174)
Key features and details
- Rabbit polyclonal to SPARC
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SPARC抗体
参阅全部 SPARC 一抗 -
描述
兔多克隆抗体to SPARC -
宿主
Rabbit -
特异性
There were no cross reactivities obtained with human Osteopontin, and human Bone Sialoprotein. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein (Human).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: Whole serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14174于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
|
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. -
序列相似性
Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain. -
发展阶段
Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair. -
细胞定位
Secreted > extracellular space > extracellular matrix > basement membrane. In or around the basement membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6678 Human
- Omim: 182120 Human
- SwissProt: P09486 Human
- Unigene: 111779 Human
- Unigene: 708558 Human
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别名
- AA517111 antibody
- Basement membrane protein 40 antibody
- Basement-membrane protein 40 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPARC antibody (ab14174)ab14174 (1/1000) staining SPARC in human substanta nigra using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and extracellular matrix.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
数据表及文件
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Datasheet download
文献 (17)
ab14174 被引用在 17 文献中.
- Luzuriaga J et al. Osteogenic differentiation of human dental pulp stem cells in decellularised adipose tissue solid foams. Eur Cell Mater 43:112-129 (2022). PubMed: 35312032
- Henry B et al. Splenic clearance of rigid erythrocytes as an inherited mechanism for splenomegaly and natural resistance to malaria. EBioMedicine 82:104167 (2022). PubMed: 35843175
- Siar CH & Ng KH Epithelial-to-mesenchymal transition in ameloblastoma: focus on morphologically evident mesenchymal phenotypic transition. Pathology 51:494-501 (2019). PubMed: 31262562
- Wang Q et al. Silencing of SPARC represses heterotopic ossification via inhibition of the MAPK signaling pathway. Biosci Rep 39:N/A (2019). PubMed: 31548362
- Khetan K et al. SPARC expression in desmoplastic and non desmoplastic pancreatic carcinoma and cholangiocarcinoma. Pathol Res Pract 215:152685 (2019). PubMed: 31727501
