AB22552

Anti-Sp7 / Osterix 抗体

Name: Sp7 / Osterix抗体 (ab22552)| Abcam中文官网
Brand: Abcam Limited
SKU: AB22552
Price: 5090 CNY
Availability: InStock
Rating: 5 (24 reviews)

Anti-Sp7 / Osterix antibody

BOND RX™ Validated
了解详情

(24 Reviews)

(504 Publications)

Anti-Sp7 / Osterix antibody (ab22552) is a rabbit polyclonal antibody detecting Sp7 / Osterix in IHC-P. Suitable for Mouse, Rat.

- Over 420 publications
- Trusted since 2006

查看别名

Osx, Sp7, Transcription factor Sp7, C22, Zinc finger protein osterix

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

IHC image of SP7/Osterix staining in a section of formalin-fixed paraffin-embedded normal rat E15 embryo performed on a Leica BOND® system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22552 at 1μg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

ab22552 staining Osterix in rat femur sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was perfusion fixed with 4% PFA in 0.1M phosphate buffer (pH 7.4) via the left ventricle and decalcified in 10% EDTA. Samples were incubated with primary antibody (1/2000) for 12 hours at 4°C. Histofine simple stain rat MAX PO was used as the secondary antibody.

This image is courtesy of an anonymous collaborator

IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemical analysis of paraffin embedded mouse osteosarcoma tissue labeling Sp7/Osterix with ab22552 at 1/1000 (overnight at 4°C). Fixed 48h in 4% formol. Decalcified in 4% EDTA and 0.2% PFA pH7.4 before inclusion in paraffin. Biotin conjugated secondary 1h RT. Amplification StreptABC, substrate DAB.

This image is courtesy of Jérôme AMIAUD, Laboratoire de Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives. We thank them for their important contribution to the validation of ab22552.

IHC-P

AbReview6355****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

The image shows Osterix staining in rat bone tibiae using ab22552 at 1/100.

This image is courtesy of an anonymous Abreview

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

IHC image of SP7/Osterix staining in a section of formalin-fixed paraffin-embedded normal mouse E15 embryo performed on a Leica BOND® system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab22552 at 1μg/ml for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat

应用

IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Antigen retrieval is not essential but may optimise staining." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Antigen retrieval is not essential but may optimise staining." } } }

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产品详情

Product Specifications
Anti-Sp7 / Osterix antibody (ab22552) is a rabbit polyclonal antibody and is validated for use in IHC-P in mouse, rat samples.
Anti-Sp7 / Osterix antibody (ab22552) specifically detects Sp7 / Osterix (UniProt ID: Q8TDD2; Molecular weight: 45kDa) and is sold in 50 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Sp7 / Osterix antibody (ab22552) has high sensitivity and specificity.
Anti-Sp7 / Osterix antibody (ab22552) has been cited over 422 times in peer reviewed journals and is trusted by the scientific community.
Anti-Sp7 / Osterix antibody (ab22552) has 23 independent reviews from customers.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional activator essential for osteoblast differentiation (PubMed : 11792318, PubMed : 17510056, PubMed : 30026585). Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (PubMed : 11792318, PubMed : 17510056).

See full target information Sp7

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文献 (504)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:35547 PubMed41073566

2025

Total flavonoids of rhizoma drynariae improved the osteogenic and pro-angiogenic capacities of BMSCs to accelerate long bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hengjun Huang,Silu Li,Huang Zhan,Fenfang Gong,Jian Liu,Zhenya Liu,Hui Li,Chengyu Yang

International journal of oral science 17:65 PubMed40998797

2025

PLAGL1-IGF2 axis regulates osteogenesis of postnatal condyle development.

Applications

Unspecified application

Species

Unspecified reactive species

Jinrui Sun,Jingyi Xu,Yue Xu,Yili Liu,Enhui Yao,Jiahui Du,Xinquan Jiang

Communications biology 8:1309 PubMed40883591

2025

Ovalbumin-induced asthma leads to bone loss with Piezo channel suppression in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weiqi Gao,Takeshi Sawada,Ailin Cao,Reiko U Yoshimoto,Yu Yamaguchi,Yukie Takahashi,Takaichi Fukuda,Yasuyoshi Ohsaki,Reona Aijima,Tomoko Kadowaki,Takayuki Tsukuba,Mizuho A Kido

BMC biology 23:268 PubMed40859304

2025

Cranial base synostosis in mice caused by upregulation of Wnt following partial inhibition of Shh.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangping Chen,Chengyan Ren,Chuanqing Mao,Yongzhen Lai,Meng Lu,Yuanjing Jiang,Weihui Chen

Frontiers in bioengineering and biotechnology 13:1639117 PubMed40837020

2025

Ipriflavone ameliorates intervertebral disc degeneration by inhibiting osteoporosis of vertebral body and pyroptosis of the nucleus pulposus in instability of lumbar spine and diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Sun,Hai-Jing Zhang,Hui Wang,Gang Ji,Ya-Heng Zhao,Gao-Cen Li,Shao-Shi Guo,Lu-Feng Lin,Yu-Jie Jin,Xue-Li Zhang,Xin-Yu Nan,Chang-Cheng Liu,Guo-Bin Liu

Frontiers in endocrinology 16:1570685 PubMed40687591

2025

Jinkui Shenqi Pill accelerates osteoporotic fracture healing by promoting bone formation through neurosensory PGE2/EP4/p-CREB axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jihao Xi,Danqing Fu,Dan Xu,Ruhan Shen,Yan Zhao,Haoqiang Dai,Chenjie Xia,Peihong Zhou

Nature communications 16:5425 PubMed40593719

2025

Protein kinase A is a dependent factor and therapeutic target in mouse models of fibrous dysplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyu Liu,Lu Xing,Wenlong Huang,Ning Ji,Hang Zhao,Qianming Chen,Xianglong Han,Ding Bai,Xuefeng Zhao

Organogenesis 21:2519649 PubMed40570323

2025

SS-31 Targets NOS2 to Enhance Osteogenic Differentiation in Aged BMSCs by Restoring Mitochondrial Function.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Duan,Qindong Zhang,Jinqiang Zhu,Jiaming Wang

Journal of orthopaedic translation 52:387-403 PubMed40476065

2025

Vitamins D and K jointly protect against osteoarthritis via regulating OSCAR during osteoclastogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhao,Qianhua Ou,Hong Huang,Delong Li,Jianmao Chen,Song Xue,Zuoqing Zhou,Guangfeng Ruan,Changhai Ding

Nature communications 16:4330 PubMed40346043

2025

Self-propagating wave drives morphogenesis of skull bones in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Yiteng Dang,Johanna Lattner,Adrian A Lahola-Chomiak,Diana Alves Afonso,Elke Ulbricht,Anna Taubenberger,Steffen Rulands,Jacqueline M Tabler

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Sp7 / Osterix 抗体

Anti-Sp7 / Osterix antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)