重组Anti-SP17抗体[EPR6497(2)] (ab181079)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6497(2)] to SP17
- Suitable for: IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SP17抗体[EPR6497(2)]
参阅全部 SP17 一抗 -
描述
兔单克隆抗体[EPR6497(2)] to SP17 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IPmore details -
种属反应性
与反应: Human
预测可用于: Cynomolgus monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human testis tissue. IP: Human testis tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6497(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181079于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/1500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
|
IP |
1/90.
For unpurified use at 1/50. |
说明 |
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IHC-P
1/1500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
IP
1/90. For unpurified use at 1/50. |
靶标
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功能
Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates. -
组织特异性
Testis and sperm specific. -
序列相似性
Contains 1 IQ domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 101865183 Cynomolgus monkey
- Entrez Gene: 53340 Human
- Omim: 608621 Human
- SwissProt: O19021 Cynomolgus monkey
- SwissProt: Q15506 Human
- Unigene: 286233 Human
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别名
- Band 34 antibody
- Cancer/testis antigen 22 antibody
- CT22 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SP17 with purified ab181079 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labeling SP17 with unpurified ab181079 at 1/250 dilution. Counterstained with hematoxylin.
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ab181079 (purified) at 1/90 immunoprecipitating SP17 in human testis tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Western blot analysis of SP17 in immunoprecipitation pellets from Human testis tissue lysate. Unpurified ab181079 was used at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500. Lane 2 shows negative control.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab181079 被引用在 1 文献中.
- Dasgeb B et al. Cancer-testis antigens as biomarkers for Merkel cell carcinoma: Pitfalls and opportunities. J Cutan Pathol 46:748-752 (2019). PubMed: 31218705