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重组RabMAb

重组Anti-SP17抗体[EP6496] (ab172626)

Submit a review Submit a question References (2)
Flow Cytometry (Intracellular) - Anti-SP17 antibody [EP6496] (ab172626)
  • Western blot - Anti-SP17 antibody [EP6496] (ab172626)
  • Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (ab172626)
  • Western blot - Anti-SP17 antibody [EP6496] (ab172626)
  • Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (ab172626)
  • Western blot - Anti-SP17 antibody [EP6496] (ab172626)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP6496] to SP17
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-SP17抗体[EP6496]
    参阅全部 SP17 一抗

  • 描述

    兔单克隆抗体[EP6496] to SP17

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), ICC/IF, WBmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human SP17 aa 1-100. The exact sequence is proprietary.
    Database link: Q15506

  • 阳性对照

    • WB: Human, mouse and rat testis lysate Flow Cyt (intra): Jurkat cells ICC/IF: Jurkat and K-562 cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab172626于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100.
ICC/IF
1/100 - 1/250.
WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
说明
Flow Cyt (Intra)
1/100.
ICC/IF
1/100 - 1/250.
WB
1/1000 - 1/10000. Predicted molecular weight: 17 kDa.

靶标

图片

  • Flow Cytometry (Intracellular) - Anti-SP17 antibody [EP6496] (ab172626)
    Flow Cytometry (Intracellular) - Anti-SP17 antibody [EP6496] (ab172626)

    Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling SP17 with purified ab172626 at 1/100 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Western blot - Anti-SP17 antibody [EP6496] (ab172626)
    Western blot - Anti-SP17 antibody [EP6496] (ab172626)
    All lanes : Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution (Purified)

    Lane 1 : Rat testis lysates
    Lane 2 : Mouse testis lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa
    Observed band size: 22-24 kDa why is the actual band size different from the predicted?



    SP17 is triplet protein what have been described in the literature PMID: 9986726.

  • Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (ab172626)
    Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (ab172626)

    Immunocytochemistry/ Immunofluorescence analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling SP17 with purified ab172626 at 1/100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-SP17 antibody [EP6496] (ab172626)
    Western blot - Anti-SP17 antibody [EP6496] (ab172626)
    Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution (Purified) + Human testis lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 17 kDa
    Observed band size: 22-24 kDa why is the actual band size different from the predicted?



    SP17 is triplet protein what have been described in the literature PMID: 9986726.

  • Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (ab172626)
    Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (ab172626)

    Immunofluorescence analysis of Jurkat cells staining SP17 with ab172626 (unpurified) at 1/100 dilution (green). DAPI nuclear staining (blue).

  • Western blot - Anti-SP17 antibody [EP6496] (ab172626)
    Western blot - Anti-SP17 antibody [EP6496] (ab172626)
    All lanes : Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution (unpurified)

    Lane 1 : Mouse testis lysate
    Lane 2 : Rat testis lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 17 kDa

文献 (2)

发表研究结果有使用 ab172626？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab172626 被引用在 2 文献中.

  • Panner Selvam MK  et al. Treatment of semen samples with a-chymotrypsin alters the expression pattern of sperm functional proteins-a pilot study. Andrology 6:345-350 (2018). PubMed: 29409138
  • Zhang Z  et al. Decitabine treatment sensitizes tumor cells to T-cell-mediated cytotoxicity in patients with myelodysplastic syndromes. Am J Transl Res 9:454-465 (2017). PubMed: 28337274

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