重组Anti-SP17抗体[EP6496]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (AB172626)

Immunocytochemistry/ Immunofluorescence analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling SP17 with purified ab172626 at 1/100 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (AB172626)

Immunofluorescence analysis of Jurkat cells staining SP17 with ab172626 (unpurified) at 1/100 dilution (green). DAPI nuclear staining (blue).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SP17 antibody [EP6496] (AB172626)

Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling SP17 with purified ab172626 at 1/100 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• WB

Lab

Western blot - Anti-SP17 antibody [EP6496] (AB172626)

SP17 is triplet protein what have been described in the literature PMID : 9986726.

All lanes:

Western blot - Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution

All lanes:

Human testis lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 17 kDa

Observed band size: 22-24 kDa

false

• WB

Lab

Western blot - Anti-SP17 antibody [EP6496] (AB172626)

SP17 is triplet protein what have been described in the literature PMID : 9986726.

All lanes:

Western blot - Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution

Lane 1:

Rat testis lysates at 15 µg

Lane 2:

Mouse testis lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 17 kDa

Observed band size: 22-24 kDa

false

• WB

Supplier Data

Western blot - Anti-SP17 antibody [EP6496] (AB172626)

All lanes:

Western blot - Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution

Lane 1:

Mouse testis lysate at 10 µg

Lane 2:

Rat testis lysate at 10 µg

Lane 3:

Human testis lysate at 10 µg

Predicted band size: 17 kDa

false