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AB308364

重组Anti-SP1抗体[HL1396]

Anti-SP1 antibody [HL1396]

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(2 Publications)

Rabbit Recombinant Monoclonal SP1 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat, Transfected cell line samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SP1.

查看别名

TSFP1, SP1, Transcription factor Sp1

6 Images
Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of human breast carcinoma tissue labeling SP1 using ab308364 at 1/500 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of human breast carcinoma tissue labeling SP1 using ab308364 at 1/500 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (AB308364)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SP1 antibody [HL1396] (AB308364)

For immunofluorescence analysis, HeLa cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of SP1 protein using ab308364 at 1/500 dilution (green). Alpha Tubulin stained by alpha Tubulin antibody (red) diluted at 1/1000

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of rat intestine, colon and lung tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of mouse intestine, colon and lung tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-SP1 antibody [HL1396] (AB308364)

Immunohistochemical analysis of mouse intestine tissue labeling SP1 using ab308364 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL1396

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Mouse, Rat

应用

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human SP1. The exact immunogen used to generate this antibody is proprietary information.

P08047

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搭配使用产品

Sample Prep & Detection Kits

AB201807

APC Conjugation Kit - Lightning-Link®

Fluorescence Microscopy - APC Conjugation Kit - Lightning-Link® (AB201807)

  • 0
  • 0
Sample Prep & Detection Kits

AB201808

Gold Conjugation Kit (10 nm, 20 OD)

Conjugation - Gold Conjugation Kit (10 nm, 20 OD) (AB201808)

  • 0
  • 0
Primary Antibodies

AB59257

Anti-SP1 (phospho T453) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP1 (phospho T453) antibody (AB59257)

  • 4
  • 6
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }
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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Also binds the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression. Isoform 3 is a stronger activator of transcription than isoform 1. Positively regulates the transcription of the core clock component BMAL1 (PubMed : 10391891, PubMed : 11371615, PubMed : 11904305, PubMed : 14593115, PubMed : 16377629, PubMed : 16478997, PubMed : 16943418, PubMed : 17049555, PubMed : 18171990, PubMed : 18199680, PubMed : 18239466, PubMed : 18513490, PubMed : 18619531, PubMed : 19193796, PubMed : 20091743, PubMed : 21046154, PubMed : 21798247). Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (By similarity).
See full target information SP1
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:737 PubMed40254586

2025

Targeting GSK3β and signaling pathways in breast cancer: role of individual members of miR- 23/24/27 cluster.

Applications

Unspecified application

Species

Unspecified reactive species

Harshi Gupta,Anushka Raghubansi, Bharat,Kritika Sharma,Krittika Zutshi,Partibha Panchal,Sushant Bhattacharya,Piyush Ranjan,Gopal Puri,Neeru Saini

Folia biologica 70:219-228 PubMed39692576

2024

Effects of Different Light Environments with Varying Spectral Composition on the Axial Lengths and Scleral Specificity Protein 1 and Collagen Type I Expression in Juvenile Guinea Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbao Yuan,Yuliang Wang,Xinyu Xu,Mei Yang,Yipeng Fan,Xiaopan Shi,Lulu Sun,Mingyu Shan,Lei Ma
View all publications
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Abcam Product Promise

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