Anti-SOX9抗体[3C10] - BSA and Azide free (ab76997)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)](https://www.abcam.cn/ps/products/76/ab76997/Images/ab76997-445039-AntiSOX9-antibody.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)")
Key features and details
- Mouse monoclonal [3C10] to SOX9 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG2a
概述
-
产品名称
Anti-SOX9抗体[3C10] - BSA and Azide free
参阅全部 SOX9 一抗 -
描述
小鼠单克隆抗体[3C10] to SOX9 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment: EQLSPSHYSE QQQHSPQQIA YSPFNLPHYS PSYPPITRSQ YDYTDHQNSS SYYSHAAGQG TGLYSTFTYM NPAQRPMYTP IADTSGVPSI PQTHSPQHWE QPVYTQLTRP, corresponding to amino acids 400-509 of human SOX9 (NP_000337) with a 26 kDa tag
-
阳性对照
- WB: HepG2 whole cell lysate (ab7900). IHC-P: Human tonsil tissue. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
-
常规说明
This product was changed from ascites to tissue culture supernatant on 22/03/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
3C10 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76997于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa.
|
|
| IHC-P | (2) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
|
| ICC/IF | (2) |
Use at an assay dependent concentration.
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 56 kDa. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
IP
Use at an assay dependent concentration. |
靶标
-
功能
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. -
疾病相关
Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage. -
序列相似性
Contains 1 HMG box DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 6662 Human
- Omim: 608160 Human
- SwissProt: P48436 Human
- Unigene: 647409 Human
-
别名
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)
Immunoperoxidase analysis of SOX9 expression on formalin fixed, paraffin embedded human tonsil tissue using 0.7 µg/ml of ab76997.
-
![Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)](https://www.abcam.cn/ps/products/76/ab76997/Images/ab76997-445038-AntiSOX9-antibody.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)Immunocytochemistry/Immunofluorescence analysis of SOX9 expression in HepG2 cells, using 10 µg/ml of ab76997.
-
![Flow Cytometry - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)](https://www.abcam.cn/ps/products/76/ab76997/Images/ab76997-92605-anti-sox9-antibody-3c10-flow-cytometry.jpg)
Flow Cytometry - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)Overlay histogram showing HepG2 cells stained with ab76997 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76997, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
-
![Western blot - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)](https://www.abcam.cn/ps/products/76/ab76997/Images/ab76997-92609-anti-sox9-antibody-3c10-western-blot.jpg)
Western blot - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 56 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
-
![Western blot - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)](https://www.abcam.cn/ps/products/76/ab76997/Images/ab76997-92608-anti-sox9-antibody-3c10-western-blot.jpg)
Western blot - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997) at 5 µg/ml + HepG2 cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaThis image was generated using the ascites version of the product.
-
![Immunoprecipitation - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)](https://www.abcam.cn/ps/products/76/ab76997/Images/ab76997-275289-anti-sox9-antibody-3c10-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SOX9 antibody [3C10] - BSA and Azide free (ab76997)Immunoprecipitation of SOX9 transfected lysate using anti-SOX9 monoclonal antibody and Protein A magnetic beads. Western blot was performed with an rabbit anti-SOX9 polyclonal antibody.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (54)
ab76997 被引用在 54 文献中.
- Jeon SB et al. CD34dim cells identified as pluripotent stem cell-derived definitive hemogenic endothelium purified using bone morphogenetic protein 4. Cell Prolif 56:e13366 (2023). PubMed: 36478274
- Xu Y et al. SOX9 and HMGB3 co-operatively transactivate NANOG and promote prostate cancer progression. Prostate 83:440-453 (2023). PubMed: 36541373
- Liang Y et al. Morphometric analysis of the size-adjusted linear dimensions of the skull landmarks revealed craniofacial dysmorphology in Mid1-cKO mice. BMC Genomics 24:68 (2023). PubMed: 36759768
- Magallón-Lorenz M et al. Deep genomic analysis of malignant peripheral nerve sheath tumor cell lines challenges current malignant peripheral nerve sheath tumor diagnosis. iScience 26:106096 (2023). PubMed: 36818284
- Dai DL et al. Human Alzheimer's disease reactive astrocytes exhibit a loss of homeostastic gene expression. Acta Neuropathol Commun 11:127 (2023). PubMed: 37533101