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Anti-SOX8抗体(ab27655)

Reviews (6)Q&A (11)References (19)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX8 antibody (ab27655)
  • Western blot - Anti-SOX8 antibody (ab27655)

Key features and details

  • Rabbit polyclonal to SOX8
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Chicken
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-SOX10 antibody [EPR4007] (ab155279)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-SOX8抗体
    参阅全部 SOX8 一抗

  • 描述

    兔多克隆抗体to SOX8

  • 宿主

    Rabbit

  • 特异性

    Antibody was raised using a synthetic peptide corresponding to Sox8. However, this antibody demonstrates cross reactivity against Sox10. Does not cross react with SOX9

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Mouse, Chicken

  • 免疫原

    Synthetic peptide corresponding to Chicken SOX8 aa 400-500 (C terminal).
    Database link: P57074

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab27655于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.
WB (2)
Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).
说明
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能

    May play a role in central nervous system, limb and facial development. May be involved in male sex determination. Binds the consensus motif 5'-[AT][AT]CAA[AT]G-3'.

  • 序列相似性

    Contains 1 HMG box DNA-binding domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • MGC24837 antibody
      • SOX 8 antibody
      • Sox8 antibody
      • SOX8_HUMAN antibody
      • SRY (sex determining region Y) box 8 antibody
      • SRY box 8 antibody
      • SRY box containing gene 8 antibody
      • Transcription factor SOX 8 antibody
      • Transcription factor SOX-8 antibody
      • Transcription factor SOX8 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX8 antibody (ab27655)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX8 antibody (ab27655)
      Sections through the trunk of E4 chick embryos fixed in 4 % paraformaldehyde and stained with anti-SOX10 (ab27655) diluted 1/5000. Secondary antibody was FITC-conjugated donkey anti-rabbit. ab27655 stains the embryonic neural crest cells which can be seen migrating into the dorsal root ganglion.
    • Western blot - Anti-SOX8 antibody (ab27655)
      Western blot - Anti-SOX8 antibody (ab27655)
      All lanes : Anti-SOX8 antibody (ab27655) at 1/500 dilution

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Heart (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 50 kDa
      Observed band size: 56 kDa why is the actual band size different from the predicted?
      Additional bands at: 100 kDa, 74 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 8 minutes


      SOX10 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (19)

    发表研究结果有使用 ab27655？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab27655 被引用在 19 文献中.

    • Mac Donald K & Iulianella A The actin-cytoskeleton associating protein BASP1 regulates neural progenitor localization in the neural tube. Genesis 60:e23464 (2022). PubMed: 34897971
    • Inubushi T  et al. The cell surface hyaluronidase TMEM2 plays an essential role in mouse neural crest cell development and survival. PLoS Genet 18:e1009765 (2022). PubMed: 35839257
    • Wang Y  et al. SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in mice. Cell Rep 41:111842 (2022). PubMed: 36543123
    • Wang Y  et al. PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination. Cell Rep 37:109695 (2021). PubMed: 34610310
    • Zhang S  et al. HIFa Regulates Developmental Myelination Independent of Autocrine Wnt Signaling. J Neurosci 41:251-268 (2021). PubMed: 33208471
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 17 Abreviews or Q&A

    Immunohistochemistry free floating abreview for Anti-SOX8 antibody

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Chicken Tissue sections (dorsal root ganglion)
    Specification
    dorsal root ganglion
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 28 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX10 antibody

     Average
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (Kidney)
    Permeabilization
    Yes - 0.1% triton x-100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 01 2016

    Western blot abreview for Anti-SOX10 antibody

     Below Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (Kiney)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Kiney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 02 2016

    Western blot abreview for Anti-SOX10 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 02 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-SOX10 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3% triton x-100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 01 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX10 antibody

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
    Specification
    Dorsal Root Ganglia (DRG) neurons
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 18 2012

    Question

    We have some doubts about the data sheat of this ab. For the specificity it is written that: "Antibody was raised using a synthetic peptide corresponding to Sox8. However, this antibody demonstrates cross reactivity against Sox10. Does not cross react with SOX9"
    Can you clarify better what you mean? Is it correct or there is a spelling error? Does it mean that the ab recognise both Sox10 and Sox8 or is it really specific for Sox10?

    Read More
    Answer



    The immunogen was actually chicken Sox8. However, we are confident that it also recognizes Sox10 and does not cross react with Sox9.

    The originator used what turns out to be the chick Sox8 sequence for the peptide. (When they originally designed the peptide the identification of chick Sox8 and Sox10 wasn't 100% clear). However, from characterization of the antibody it definitely recognizes Sox10 in both mouse and chick tissue, this is not too surprising as the genes are highly related. It doesn't cross react, at least in assays in either chick or mouse, with Sox9 - another SoxE family member. It probably also recognizes Sox8 however we're not aware of any tissue that expresses Sox8 in the absence of Sox10, so its not possible to test this directly.

    Read More

    Question

    Bonjour,
    Merci de votre réponse.
    Nous serions éventuellement intéressé par un autre lot de ab2765 s’il provient d’un lapin dont le sérum vous a permis d’obtenir le marquage que vous présentez dans la fiche technique.
    Par contre si les lots que vous avez en stock n’ont pas été testé en Immunohistochimie par votre service qualité nous préférions bénéficier d’un avoir.
    Je reste à votre disposition si vous avez besoin d’informations complémentaires. J’ai notamment oublié de vous préciser lors de mon précédent mail que l’anticorps avait bien été aliquoté et conservé à -20°C après sa réception.

    Read More
    Answer

    Merci pour votre email.

    J'ai mis en place l'envoi d'une nouvelle unité de ab27655, le numéro de commande de remplacement gratuit de ce produit est ***.
    Par mesure préventive, j'ai pris l'initiative de retarder l'envoi du ab72876 à lundi afin qu'il ne reste pas en transit tout le weekend. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

    Veuillez noter que cet anticorps de remplacement est également couvert par la garantie Abpromise, donc n'hésitez pas à nous contacter de nouveau si nécessaire.

    Read More

    Question

    Product code: 27655
    Lot number: GR93638-1

    Inquiry:
    Hello,
    We purchased your anti-Sox-10 antibody (ab27655) to label neural cell crest on cryostat section of chick embryos (E3.5 to E4.5).
    Unfortunately, we were not able to get the nice labelling that is shown on the datasheet.
    We tried it on embryos fixed in 4% PFA for either 2h or overnight at 4°c, with different permeabilisation (triton 0,5% or 0,1%), blocking solutions (horse serum or BSA) and antibody concentrations (1/1000 to 1/100).
    The secondary antibody was an anti-rabbit Alexa 555 1/400 and the washes were performed in PBS 1X (two washes of 20 minutes after the primary and secondary antibody).
    Although, the labelling seems better with a 1h30 pre-blocking/premeabilisation in BSA 6% + 0,5% triton and an overnight incubation of the antibody diluted in BSA 1% +/- 0.1%Triton, it is still really weak.
    Thus, I would like to know if you have any suggestions to improve you IHC or if you could provide us some more detailed description of the procedure used to get the labelling you show?
    Thanks

    Read More
    Answer

    Thank you for contacting us.

    I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

    I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.
    If a replacement is a better resolution for you, I can offer:

    one unit of a different lot of ab27655
    one unit of anti-SOX10 ab107532 which shows 87% homology with the chicken protein, www.abcam.com/ab107532
    one unit of anti-SOX10 ab64055 which also shows 87% homology with the chicken protein, www.abcam.com/ab64055




    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed. Could you also please confirm the name and address of your lab?

    Read More

    Question

    Hello, A collegue contact you on July 30th for a problem with antibody which stay at room temperature during a hot week end. I tested this antibody on spinal columns of P0 mouse with the same protocol published by Mukhopadhyay A et al. HeyL regulates the number of TrkC neurons in dorsal root ganglia. Dev Biol 334:142-51 (2009). IHC-Fr Mouse. I tested, also, several dilution for this antibody. Unfortunately, the antibody does not work. Can you replace it ? Sincerely yours,

    Read More
    Answer

    Thank you for contacting us.

    I am very sorry for the inconveniences and am happy to create a free of charge replacement order in compensation for the problems experienced with this antibody. The order number is XXXXXX. You should receive an automatic confirmation. To check the status of the order please contact our Customer Service team and reference this number.

    I hope this new vial works as expected. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    1-10 of 17 Abreviews or Q&A

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