Anti-SOX6抗体(ab125196)
Key features and details
- Rabbit polyclonal to SOX6
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SOX6抗体
参阅全部 SOX6 一抗 -
描述
兔多克隆抗体to SOX6 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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免疫原
Synthetic peptide, corresponding to a region within amino acids 778-828 of Human SOX6 (EAW68458.1).
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阳性对照
- 293T, HeLa and Jurkat whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab125196 was affinity purified using an epitope specific to SOX6 immobilized on a solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125196于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 92 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 92 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Transcriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation. -
组织特异性
Expressed in a wide variety of tissues, most abundantly in skeletal muscle. -
序列相似性
Contains 1 HMG box DNA-binding domain. -
翻译后修饰
Sumoylation inhibits the transcriptional activity. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 55553 Human
- Entrez Gene: 100172098 Orangutan
- Omim: 607257 Human
- SwissProt: P35712 Human
- SwissProt: Q5RCU4 Orangutan
- Unigene: 368226 Human
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别名
- HSSOX 6 antibody
- HSSOX6 antibody
- SOX 6 antibody
see all
图片
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Western blot - Anti-SOX6 antibody (ab125196)All lanes : Anti-SOX6 antibody (ab125196) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 92 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-SOX6 antibody (ab125196)Detection of SOX6 by Western Blot of Immunprecipitate.
ab125196 at 1µg/ml staining SOX6 in HeLa whole cell lysate immunoprecipitated using ab125196 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab125196 被引用在 1 文献中.
- Leow SC et al. The transcription factor SOX6 contributes to the developmental origins of obesity by promoting adipogenesis. Development 143:950-61 (2016). PubMed: 26893351