Anti-SOX30抗体(ab272553)
Key features and details
- Rabbit polyclonal to SOX30
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SOX30抗体 -
描述
兔多克隆抗体to SOX30 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Macaque monkey
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免疫原
Recombinant fragment corresponding to Human SOX30 aa 450-600.
Database link: O94993 -
阳性对照
- WB: RT4 and U-251 MG cells. IHC-P: Human testis tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272553于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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相关性
SOX30 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. This protein may be involved in the differentiation of developing male germ cells. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 11063 Human
- Omim: 606698 Human
- SwissProt: O94993 Human
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别名
- Sex determining region Y box 30 antibody
- Sox30 protein type II antibody
- SRY box 30 antibody
see all
图片
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Western blot - Anti-SOX30 antibody (ab272553)Lanes 2-3 : Anti-SOX30 antibody (ab272553) at 0.4 µg/ml
Lane 1 : Molecular Weight ladder
Lane 2 : RT4 cells (Human urinary bladder cancer cell line)
Lane 3 : U-251 MG cells (Human brain glioma cell line) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX30 antibody (ab272553)Paraffin-embedded formalin-fixed human testis tissue stained for SOX30 using ab272553 at 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-SOX30 antibody (ab272553)All lanes : Anti-SOX30 antibody (ab272553) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK293T lysate (Human epithelial cell line from embryonic kidney transformed with large T antigen)
Lane 2 : SOX30 over-expression HEK293T lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX30 antibody (ab272553)Paraffin-embedded formalin-fixed human liver tissue stained for SOX30 using ab272553 at 1/50 dilution in immunohistochemical analysis. No positive staining as expected.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272553 尚未被引用在任何文献中。