Anti-SOX10 抗体 [EPR4007]

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-SOX10 antibody [EPR4007] (AB155279)

Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab155279 at 1/50 (2.2 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control : PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [EPR4007] (AB155279)

Immunocytochemistry/ Immunofluorescence analysis of C6 (Rat glial tumor glial cell) cells labeling SOX10 with purified ab155279 at 1 : 50 dilution (3.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SOX10 antibody [EPR4007] (AB155279)

Intracellular Flow Cytometry analysis ofA-375 (human malignant melanoma cell line) cells labeling with purified ab155279 at 1/200 dilution (1ug/ml) (Red). Cells were fixed with4% paraformaldehydeand permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody.Rabbit monoclonal IgG (Black) (ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

• WB

Lab

Western blot - Anti-SOX10 antibody [EPR4007] (AB155279)

The bands observed are consistent with what have been described in PMID : 21423190

All lanes:

Western blot - Anti-SOX10 antibody [EPR4007] (ab155279) at 1/1000 dilution

Lane 1:

Human brain lysates at 20 µg

Lane 2:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

Mouse brain lysates at 20 µg

Lane 4:

Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg

Lane 5:

Rat brain lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 49 kDa

Observed band size: 56-75 kDa

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• WB

Supplier Data

Western blot - Anti-SOX10 antibody [EPR4007] (AB155279)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-SOX10 antibody [EPR4007] (ab155279) at 1/2000 dilution

All lanes:

A375 (Human malignant melanoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

Observed band size: 56-75 kDa

false

Exposure time: 3s

• WB

CiteAb

Western blot - Anti-SOX10 antibody [EPR4007] (AB155279)

SOX10 western blot using anti-SOX10 antibody [EPR4007] ab155279. Publication image and figure legend from Miyamoto, Y., Torii, T., et al., 2016, Nat Commun, PubMed 27876794.

ab155279 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab155279 please see the product overview.

Knockout of VCAM1 in oligodendrocytes decreases myelin marker protein expression in mice.(a,b) MBP-Cre-driven VCAM1 conditional knockout (VCAM1fl/fl; Mbp-Cre) and control mouse spinal cord cross sections at postnatal day 7 were stained with an antibody against GFAP (green) or NeuN (red). Data are representative. Scale bar, 200 μm (a); 100 μm (b). (c) The intensity of GFAP staining per one square millimetre was semi-quantified at postnatal days 7, 14 and 21. Data were evaluated using Student’s t-test (non-significance; n=6 slices of two independent experiments). (d) The number of NeuN+ cells per field was counted. Data were evaluated using Student’s t-test (non-significance; n=5 slices of two independent experiments). (e) Spinal cord cross sections of VCAM1fl/fl; Mbp-Cre and control mice at postnatal day 7 were co-stained with an antibody against CC1 (red) and DAPI (blue). Data are representative. Scale bar, 100 μm. (f,g) Antibodies against CC1 (red) and Olig2 (green) were used for co-staining in spinal cord cross sections at postnatal days 7, 14, 21 and 28. Data are representative. Scale bar, 100 μm. The percentage of CC1+ cells among the Olig2+ cells is shown. Data were evaluated using Student’s t-test (**P=0.00638 (P7), 0.000961 (P14), 0.00111 (P21) or 2.11E-09 (P28); n=6–10 slices of two independent experiments). (h,i) Corpus callosum sections of VCAM1fl/fl; Mbp-Cre and control mouse (h) or VCAM1fl/fl; Ng2-Cre and control mouse (i) at postnatal day 11 were co-stained with an anti-MBP antibody (red) and DAPI (blue). Data are representative of six slices of two independent experiments. Scale bar, 200 μm. (j) Tissue lysates from 7-day-old spinal cords or 11-day-old brains were immunoblotted with an antibody against MBP, CNPase, VCAM1, CD69, MAG, Olig2, Sox10, Ascl1, Nkx2.1 or actin. Data are representative of three experiments.

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