重组Anti-SOX1抗体[EPR4766] (ab109290)
![Western blot - Anti-SOX1 antibody [EPR4766] (ab109290)](https://www.abcam.cn/ps/products/109/ab109290/Images/ab109290-478752-anti-sox1-antibody-epr4766-westernblot-human-mouse-rat.jpg "Western blot - Anti-SOX1 antibody [EPR4766] (ab109290)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4766] to SOX1
- Suitable for: WB, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SOX1抗体[EPR4766]
参阅全部 SOX1 一抗 -
描述
兔单克隆抗体[EPR4766] to SOX1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, Flow Cyt (Intra)more details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4766 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109290于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
|
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| ICC/IF | (1) |
1/100.
|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
|
ICC/IF
1/100. |
|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Transcriptional activator. -
组织特异性
Mainly expressed in the developing central nervous system. -
序列相似性
Contains 1 HMG box DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6656 Human
- Entrez Gene: 20664 Mouse
- Omim: 602148 Human
- SwissProt: O00570 Human
- SwissProt: P53783 Mouse
- Unigene: 202526 Human
- Unigene: 39088 Mouse
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别名
- Sex determining region Y box 1 antibody
- sox1 antibody
- SOX1_HUMAN antibody
see all
图片
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Western blot - Anti-SOX1 antibody [EPR4766] (ab109290)All lanes : Anti-SOX1 antibody [EPR4766] (ab109290) at 1/1000 dilution (Purified)
Lane 1 : NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 2 : Mouse brain lysate
Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
Lane 4 : Rat cerebral cortex lysate
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 39 kDa -
![Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] (ab109290)](https://www.abcam.cn/ps/products/109/ab109290/Images/ab109290-8-anti-sox1-antibody-epr4766-flowcytometry-nccit-human.jpg)
Flow Cytometry (Intracellular) - Anti-SOX1 antibody [EPR4766] (ab109290)Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labelling SOX1 with Purified ab109290 at 1:100 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (ab109290)](https://www.abcam.cn/ps/products/109/ab109290/Images/ab109290-7-anti-sox1-antibody-epr4766-immunocytochemistry-nccit-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (ab109290)Immunocytochemistry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling SOX1 with Purified ab109290 at 1:500 dilution (2 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Anti-SOX1 antibody [EPR4766] (ab109290)](https://www.abcam.cn/ps/products/109/ab109290/Images/ab109290-5-benefits-of-recombinant-antibodies.png)
Anti-SOX1 antibody [EPR4766] (ab109290)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab109290 被引用在 20 文献中.
- Alawad AO et al. Effects of Rhazya Stricta plant organic extracts on human induced pluripotent stem cells derived neural stem cells. PLoS One 18:e0288032 (2023). PubMed: 37478090
- Lei XX et al. SOX1 acts as a tumor hypnotist rendering nasopharyngeal carcinoma cells refractory to chemotherapy. Cell Death Discov 9:194 (2023). PubMed: 37369660
- Zhang M et al. Neural stemness unifies cell tumorigenicity and pluripotent differentiation potential. J Biol Chem 298:102106 (2022). PubMed: 35671824
- Tang PC et al. Characterization of an induced pluripotent stem cell line (UMi040-A) bearing an auditory neuropathy spectrum disorder-associated variant in TMEM43. Stem Cell Res 61:102758 (2022). PubMed: 35364395
- Luo C et al. CircFBXW7 inhibits the tumorigenesis of T-cell acute lymphoblastic leukemia through modulating miR-494-3p/SOX1 axis. Cell Death Discov 8:256 (2022). PubMed: 35538053