Name: Anti-SOS1 antibody [EPR7480]
Brand: RabMAb
SKU: ab140621
Rating: 5 (3 reviews)

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Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7480] to SOS1
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Human

Carrier Free

产品名称

Anti-SOS1抗体[EPR7480]
参阅全部 SOS1 一抗
描述

兔单克隆抗体[EPR7480] to SOS1
宿主

Rabbit
经测试应用

适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP
种属反应性

与反应: Human
预测可用于: Mouse, Rat
免疫原

Recombinant fragment corresponding to Human SOS1 aa 1050-1200.
阳性对照
- Raji, K562, HeLa and THP1 cell lysates; Human ovarian carcinoma tissue; Raji cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at -20ºC.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR7480
同种型

IgG
研究领域

Alternative Versions
- Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
Recombinant Protein
- Recombinant Human SOS1 protein (His tag) (ab233594)

The Abpromise guarantee

Abpromise™承诺保证使用ab140621于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(1)	1/1000 - 1/10000. Predicted molecular weight: 152 kDa.
IHC-P	(2)	1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/250 - 1/500.

说明
WB 1/1000 - 1/10000. Predicted molecular weight: 152 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.

应用说明

Is unsuitable for Flow Cyt or IP.

功能

Promotes the exchange of Ras-bound GDP by GTP.
组织特异性

Expressed in gingival tissues.
疾病相关

Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1) [MIM:135300]; also known as GINGF1. Gingival fibromatosis is a rare overgrowth condition characterized by a benign, slowly progressive, nonhemorrhagic, fibrous enlargement of maxillary and mandibular keratinized gingiva. GGF1 is usually transmitted as an autosomal dominant trait, although sporadic cases are common.
Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4) [MIM:610733]. NS4 is an autosomal dominant disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS4 is associated with juvenile myelomonocytic leukemia (JMML). SOS1 mutations engender a high prevalence of pulmonary valve disease; atrial septal defects are less common.
序列相似性

Contains 1 DH (DBL-homology) domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 PH domain.
Contains 1 Ras-GEF domain.
Target information above from: UniProt accession Q07889 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 6654 Human
- Entrez Gene: 20662 Mouse
- Entrez Gene: 313845 Rat
- Omim: 182530 Human
- SwissProt: Q07889 Human
- SwissProt: Q62245 Mouse
- Unigene: 709893 Human
- Unigene: 360004 Mouse
- Unigene: 60975 Mouse
see all
别名
- alternate SOS1 antibody
- GF1 antibody
- GGF1 antibody
- GINGF antibody
- gingival fibromatosis antibody
- gingival fibromatosis hereditary 1 antibody
- Guanine nucleotide exchange factor antibody
- HGF antibody
- NS4 antibody
- Son of sevenless homolog 1 (Drosophila) antibody
- Son of sevenless homolog 1 antibody
- SOS Ras/Rac guanine nucleotide exchange factor 1 antibody
- SOS-1 antibody
- Sos1 antibody
- SOS1_HUMAN antibody
see all

Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)

All lanes : Anti-SOS1 antibody [EPR7480] (ab140621) at 1/1000 dilution

Lane 1 : Wild-type A549 cell lysate
Lane 2 : SOS1 knockout A549 cell lysate
Lane 3 : HEK-293 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 152 kDa
Observed band size: 165 kDa why is the actual band size different from the predicted?

Anti-SOS1 antibody [EPR7480] (ab140621) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab140621 was shown to bind specifically to SOS1. A band was observed at 165 kDa in wild-type A549 cell lysates with no signal observed at this size in SOS1 knockout cell line. To generate this image, wild-type and SOS1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)

All lanes : Anti-SOS1 antibody [EPR7480] (ab140621) at 1/1000 dilution

Lane 1 : Wild-type A431 cell lysate
Lane 2 : SOS1 knockout A431 cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : SK-OV-3 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 152 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?

False colour image of Western blot: Anti-SOS1 antibody [EPR7480] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab140621 was shown to bind specifically to SOS1. A band was observed at 171 kDa in wild-type A431 cell lysates with no signal observed at this size in SOS1 knockout cell line ab276087 (knockout cell lysate ab283833). To generate this image, wild-type and SOS1 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Twee^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 Â°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)

All lanes : Anti-SOS1 antibody [EPR7480] (ab140621) at 1/1000 dilution

Lane 1 : Raji cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : THP1 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 152 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labelling SOS1 with ab140621 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunofluorescent staining of Raji cells labelling SOS1 with ab140621 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-SOS1 antibody [EPR7480] (ab140621)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

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Datasheet download

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发表研究结果有使用 ab140621？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab140621 被引用在 8 文献中.

Ye J et al. The Silkworm Carboxypeptidase Inhibitor Prevents Gastric Cancer Cells' Proliferation through the EGF/EGFR Signaling Pathway. Int J Mol Sci 24:N/A (2023). PubMed: 36674593
Waninger JJ et al. Biochemical characterization of the interaction between KRAS and Argonaute 2. Biochem Biophys Rep 29:101191 (2022). PubMed: 34988297
Bian Y et al. Development of SOS1 Inhibitor-Based Degraders to Target KRAS-Mutant Colorectal Cancer. J Med Chem 65:16432-16450 (2022). PubMed: 36459180
Cowan JR et al. SOS1 Gain-of-Function Variants in Dilated Cardiomyopathy. Circ Genom Precis Med 13:e002892 (2020). PubMed: 32603605
Mishra M et al. Adaptor Protein p66Shc: A Link Between Cytosolic and Mitochondrial Dysfunction in the Development of Diabetic Retinopathy. Antioxid Redox Signal 30:1621-1634 (2019). PubMed: 30105917
Mitsuda Y et al. RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci Rep 8:6423 (2018). PubMed: 29686309
Naidu S et al. PDGFR-modulated miR-23b cluster and miR-125a-5p suppress lung tumorigenesis by targeting multiple components of KRAS and NF-kB pathways. Sci Rep 7:15441 (2017). PubMed: 29133857
Baruzzi A et al. Sos1 Regulates Macrophage Podosome Assembly and Macrophage Invasive Capacity. J Immunol 195:4900-12 (2015). PubMed: 26447228

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1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (A549 tumor)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate pH6

Permeabilization

Specification

A549 tumor

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 03 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Prostate cancer)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH9 target retrieval Dako

Permeabilization

Specification

Prostate cancer

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 01 2020

Application

Western blot

Sample

Human Cell lysate - other (HeLa and HEK293E)

Gel Running Conditions

Reduced Denaturing (3-8% Tris acetate)

Loading amount

3e+007 cells

Treatment

EGF and an inhibitor

Specification

HeLa and HEK293E

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Stacy Wilson

Verified customer

提交于 Dec 19 2019

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Key features and details

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概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (8)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOS1 antibody [EPR7480]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOS1 antibody [EPR7480]

Western blot abreview for Anti-SOS1 antibody [EPR7480]