Anti-SORBS1抗体(ab224129)
Key features and details
- Rabbit polyclonal to SORBS1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SORBS1抗体
参阅全部 SORBS1 一抗 -
描述
兔多克隆抗体to SORBS1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human SORBS1 aa 250-450.
Database link: Q9BX66 -
阳性对照
- WB: Human tonsil tissue lysate. IHC-P: Human heart muscle, lymph node, cerebral cortex, colon and testis tissue. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224129于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 69,77,87,90,91,95,101,112,127,139,142,143 kDa.
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| 说明 |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 69,77,87,90,91,95,101,112,127,139,142,143 kDa. |
靶标
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功能
Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions. -
组织特异性
Detected in skeletal muscle (at protein level). Widely expressed with highest levels in heart and skeletal muscle. -
序列相似性
Contains 3 SH3 domains.
Contains 1 SoHo domain. -
翻译后修饰
O-glycosylated. -
细胞定位
Cell junction > adherens junction. Cell membrane. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with actin stress fibers. Also detected at the plasma membrane and in neuronal intranuclear inclusions. Colocalized with PXN at focal adhesions during myogenic differentiation. - Information by UniProt
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数据库链接
- Entrez Gene: 10580 Human
- Omim: 605264 Human
- SwissProt: Q9BX66 Human
- Unigene: 38621 Human
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别名
- c-Cbl-associated protein antibody
- CAP antibody
- CBL Associated Protein antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-SORBS1 antibody (ab224129)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for SORBS1 (green) using ab224129 at 4 µg/ml in ICC/IF.
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Western blot - Anti-SORBS1 antibody (ab224129)All lanes : Anti-SORBS1 antibody (ab224129) at 0.4 µg/ml
Lane 1 : Caco-2 cells lysates transfected with control siRNA
Lane 2 : Caco-2 cells lysates transfected with target specific siRNA probe #1
Lane 3 : Caco-2 cells lysates transfected with target specific siRNA probe #2
Predicted band size: 69,77,87,90,91,95,101,112,127,139,142,143 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab224129)Paraffin-embedded human colon tissue stained for SORBS1 using ab224129 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab224129)Paraffin-embedded human lymph node tissue stained for SORBS1 using ab224129 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab224129)Paraffin-embedded human testis tissue stained for SORBS1 using ab224129 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab224129)Paraffin-embedded human cerebral cortex tissue stained for SORBS1 using ab224129 at 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SORBS1 antibody (ab224129)Paraffin-embedded human heart muscle tissue stained for SORBS1 using ab224129 at 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-SORBS1 antibody (ab224129)Anti-SORBS1 antibody (ab224129) at 1/500 dilution + human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 69,77,87,90,91,95,101,112,127,139,142,143 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab224129 被引用在 4 文献中.
- Wang N et al. Development and Validation of a Prognostic Classifier Based on Lipid Metabolism-Related Genes for Breast Cancer. J Inflamm Res 15:3477-3499 (2022). PubMed: 35726216
- Liu L et al. Myocardin regulates exon usage in smooth muscle cells through induction of splicing regulatory factors. Cell Mol Life Sci 79:459 (2022). PubMed: 35913515
- Jin X et al. miR-223-3p carried by cancer-associated fibroblast microvesicles targets SORBS1 to modulate the progression of gastric cancer. Cancer Cell Int 22:96 (2022). PubMed: 35193596
- Swärd K et al. Identification of the intermediate filament protein synemin/SYNM as a target of myocardin family coactivators. Am J Physiol Cell Physiol 317:C1128-C1142 (2019). PubMed: 31461342