重组Anti-SON/DBP5抗体[EPR28617-69] (ab317311)
![Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)](https://www.abcam.cn/ps/products/317/ab317311/Images/ab317311-539765-anti-sondbp5-antibody-epr28617-69-western-blot-hela-human-cervix-adenocarcinoma-epithelial-cell-transfected-with-scrambled-sirna-control-whole-cell-lysate-40-ug.jpg "Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28617-69] to SON/DBP5
- Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SON/DBP5抗体[EPR28617-69]
参阅全部 SON/DBP5 一抗 -
描述
兔单克隆抗体[EPR28617-69] to SON/DBP5 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, Flow Cyt (Intra), WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, HeLa transfected with siRNA specifically targeti SON whole, A431, A431 whole, HeLa and 293T lysates. IHC-P: Human testis and Human colon carcinoma tissues. ICC: A431 cells. Flow Cyt: A431 cell.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28617-69 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317311于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/2000.
|
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| Flow Cyt (Intra) |
1/500.
|
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| WB |
1/1000.
|
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
ICC/IF
1/2000. |
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000. |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Represses hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Binds to the consensus DNA sequence: 5'-GA[GT]AN[CG][AG]CC-3'. Might protect cells from apoptosis. Might be involved in pre-mRNA splicing. -
组织特异性
Widely expressed, with the higher expression seen in leukocyte and heart. -
序列相似性
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 G-patch domain. -
结构域
Contains 8 types of repeats which are distributed in 3 regions. -
细胞定位
Nucleus speckle. Colocalizes with the pre-mRNA splicing factor SFRS2/SC-35. - Information by UniProt
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数据库链接
- Entrez Gene: 6651 Human
- Omim: 182465 Human
- SwissProt: P18583 Human
- Unigene: 517262 Human
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别名
- BASS1 antibody
- Bax antagonist selected in saccharomyces 1 antibody
- DBP-5 antibody
see all
图片
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Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)All lanes : Anti-SON/DBP5 antibody [EPR28617-69] (ab317311) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting SON whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 264 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
SON can undergo degradation easily.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:18952841, 27545680).
In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.
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![Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)](https://www.abcam.cn/ps/products/317/ab317311/Images/ab317311-539766-anti-sondbp5-antibody-epr28617-69-western-blot-a431-human-epidermoid-carcinoma-epithelial-cell-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)All lanes : Anti-SON/DBP5 antibody [EPR28617-69] (ab317311) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 264 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
SON can undergo degradation easily.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:18952841, 27545680).
Lysates of lanes 2-4 were freshly made and used for Western Blotting immediately to minimize protein degradation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)](https://www.abcam.cn/ps/products/317/ab317311/Images/ab317311-2-anti-sondbp5-antibody-epr28617-69-immunohistochemistry-human-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling SON/DBP5 with ab317311 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human testis. The section was incubated with ab317311 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)](https://www.abcam.cn/ps/products/317/ab317311/Images/ab317311-3-anti-sondbp5-antibody-epr28617-69-immunohistochemistry-human-colon-carcinom.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling SON/DBP5 with ab317311 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human colon carcinoma. The section was incubated with ab317311 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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![Immunocytochemistry/ Immunofluorescence - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)](https://www.abcam.cn/ps/products/317/ab317311/Images/ab317311-1-anti-sondbp5-antibody-epr28617-69-immunocytochemistry-a431.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 (human epidermoid carcinoma epithelial cell) cells labelling SON/DBP5 with ab317311 at 1/2000 (0.254 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing nuclear staining in A431 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
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![Flow Cytometry (Intracellular) - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)](https://www.abcam.cn/ps/products/317/ab317311/Images/ab317311-4-anti-sondbp5-antibody-epr28617-69-flow-cytometry-a431.jpg)
Flow Cytometry (Intracellular) - Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A431(human epidermoid carcinoma epithelial cell) cells labelling SON/DBP5 with ab317311 at 1/500 dilution (0.1ug)/Red (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
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![Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)](https://www.abcam.cn/ps/products/317/ab317311/Images/ab317311-5-benefitsofrecombinantantibodies.jpg)
Anti-SON/DBP5 antibody [EPR28617-69] (ab317311)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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