Anti-SON/DBP5抗体(ab121759)
Key features and details
- Rabbit polyclonal to SON/DBP5
- Suitable for: ICC, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SON/DBP5抗体
参阅全部 SON/DBP5 一抗 -
描述
兔多克隆抗体to SON/DBP5 -
宿主
Rabbit -
经测试应用
适用于: ICC, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human SON/DBP5 aa 1463-1598.
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阳性对照
- IHC-P: Human small intestine, tonsil, prostate, and cerebellum tissue. ICC: U-251 MG cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121759于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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ICC
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Represses hepatitis B virus (HBV) core promoter activity and transcription of HBV genes and production of HBV virions. Binds to the consensus DNA sequence: 5'-GA[GT]AN[CG][AG]CC-3'. Might protect cells from apoptosis. Might be involved in pre-mRNA splicing. -
组织特异性
Widely expressed, with the higher expression seen in leukocyte and heart. -
序列相似性
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 G-patch domain. -
结构域
Contains 8 types of repeats which are distributed in 3 regions. -
细胞定位
Nucleus speckle. Colocalizes with the pre-mRNA splicing factor SFRS2/SC-35. - Information by UniProt
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数据库链接
- Entrez Gene: 6651 Human
- Omim: 182465 Human
- SwissProt: P18583 Human
- Unigene: 517262 Human
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别名
- BASS1 antibody
- Bax antagonist selected in saccharomyces 1 antibody
- DBP-5 antibody
see all
图片
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Paraffin-embedded human small intestine tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong postiivty in nuclear speckles in glandular cells.
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ab121759, at 4 µg/ml, staining SON/DBP5 (green) in PFA-fixed, Triton X-100 permbabilized U-251 MG cells, by Immunofluorescence.
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Paraffin-embedded human prostate tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in glandular cells.
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Paraffin-embedded human tonsil tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in germinal center cells.
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Paraffin-embedded human cerebellum tissue staining SON/DBP5 with ab121759 at 1/200 dilution in immunohistochemical analysis. Moderate to strong positivity in nuclear speckles in Purkinje cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab121759 被引用在 7 文献中.
- Sandlin CW et al. Epithelial cell size dysregulation in human lung adenocarcinoma. PLoS One 17:e0274091 (2022). PubMed: 36201559
- Alexander KA et al. p53 mediates target gene association with nuclear speckles for amplified RNA expression. Mol Cell 81:1666-1681.e6 (2021). PubMed: 33823140
- Hoboth P et al. Dual-color dSTORM imaging and ThunderSTORM image reconstruction and analysis to study the spatial organization of the nuclear phosphatidylinositol phosphates. MethodsX 8:101372 (2021). PubMed: 34430268
- Fáberová V et al. Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome. Cells 9:N/A (2020). PubMed: 32403279
- Alkalay E et al. The Sub-Nuclear Localization of RNA-Binding Proteins in KSHV-Infected Cells. Cells 9:N/A (2020). PubMed: 32854341
- Kim JH et al. SON haploinsufficiency causes impaired pre-mRNA splicing of CAKUT genes and heterogeneous renal phenotypes. Kidney Int 95:1494-1504 (2019). PubMed: 31005274
- Park R & Miller G Epstein-Barr Virus-Induced Nodules on Viral Replication Compartments Contain RNA Processing Proteins and a Viral Long Noncoding RNA. J Virol 92:N/A (2018). PubMed: 30068640