Anti-Somatostatin Receptor 2 抗体 [UMB1] - C-terminal

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling Somatostatin Receptor 2 with ab134152 at 1/2000 dilution (0.928 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at a ready to use dilution. Positive staining on human breast carcinoma. The section was incubated with ab134152 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at a ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human pancreas labelling somatostatin receptor 2 with ab134152 at a concentration of 0.2µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab134152 anti somatostatin receptor 2 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)

Immunohistochemical analysis of paraffin embedded human brain tissue labeled with ab134152 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)

Immunohistochemical analysis of paraffin embedded human pancreas tissue labeled with ab134152 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)

Immunohistochemical analysis of paraffin-embedded human cervical cancer xenograft tissue (cells derived from liver metastases) using ab134152 at 1/100 dilution.

Somatostatin Receptor 2 is detected using ab134152.

From Figure 1 of Yuan et al.

Yuan et al Sci Rep. 2017; 7: 45617. Published online 2017 Apr 5. doi: 10.1038/srep45617. Reproduced under the Creative Commons Licence http://creativecommons.org/licenses/by/4.0/

• WB

Lab

Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time :
Lane 1 and 2 : 1 second.
Lane 3 and 4 : 5 seconds.

All lanes:

Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

Lane 1:

Human brain lysates prepared in RIPA lysis method at 15 µg

Lane 2:

Human brain lysates prepared in 1% SDS hot lysis method at 15 µg

Lane 3:

Mouse brain lysates prepared in RIPA lysis method at 15 µg

Lane 4:

Mouse brain lysates prepared in 1% SDS hot lysis method at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 73 kDa

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• WB

Lab

Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)

Blocking and diluting buffer : 5% NFDM/TBST.

This antibody doesn't work in MCF-7 and MDA-MB-231 cells which should be positive (PMID : 16519802). A549 cell is Somatostatin Receptor 2 negative cell line (PMID : 19148511).

All lanes:

Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

Lane 1:

U937 (Human histiocytic lymphoma monocyte) whole cell lysates prepared in RIPA lysis method at 15 µg

Lane 2:

U937 (Human histiocytic lymphoma monocyte) whole cell lysates prepared in 1% SDS hot lysis method at 15 µg

Lane 3:

MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 15 µg

Lane 4:

MCF-7 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS hot lysis method at 15 µg

Lane 5:

MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 15 µg

Lane 6:

MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysates prepared in 1% SDS hot lysis method at 15 µg

Lane 7:

A549 (Human lung carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 73 kDa

false

Exposure time: 3min

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)

Tissue Microarrays stained for Anti-Somatostatin Receptor 2 antibody [UMB1] using ab134152 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negaive (cross mark) staining per sample type tested.The sections were incubated with ab134152 for 30 mins at room temperature used at 1 : 2000 dilution (0.928 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody. Counterstain was Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• WB

Unknown

Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)

All lanes:

Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152) at 1/1000 dilution

Lane 1:

U937 (Human histiocytic lymphoma cell line) cell lysates at 10 µg

Lane 2:

INS-1 cell lysates at 10 µg

Lane 3:

Somatostatin Receptor 2 transfected HEK-293 (Human epithelial cell line from embryonic kidney) cell lysates at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 41 kDa

false

• WB

CiteAb

Western blot - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)

Somatostatin Receptor 2 western blot using anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal ab134152. Publication image and figure legend from Zhang, L., Vines, D. C., et al., 2017, Contrast Media Mol Imaging, PubMed 29097943.

ab134152 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab134152 please see the product overview.

mRNA expression level and Western blot analyses of SSTR2 and NET. (a) RNA was isolated from different neuroblastoma cell lines, converted into cDNA, followed by RT-PCR with SSTR2 and NET specific primers. The GAPDH gene was used as a reference gene. (b) Protein lysates were prepared from different neuroblastoma cell lines. Protein samples were separated by polyacrylamide gel electrophoresis. Expression of SSTR2 and NET proteins was visualized using specific antibodies. β-Actin was used as internal loading control.

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