重组Anti-Somatostatin Receptor 2抗体[UMB1] - BSA and Azide free (ab271907)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)](https://www.abcam.cn/ps/products/271/ab271907/Images/ab271907-529194-anti-Somatostatin-Receptor-2-antibody-UMB1-IHC-pancreas-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - BSA and Azide free
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Somatostatin Receptor 2抗体[UMB1] - BSA and Azide free
参阅全部 Somatostatin Receptor 2 一抗 -
描述
兔单克隆抗体[UMB1] to Somatostatin Receptor 2 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IHC-Fr -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab134152) -
阳性对照
- WB: Human and mouse brain tissue lysate. U937, INS-1 and Somatostatin Receptor 2 transfected HEK-293 cell lysates. IHC-P: Human pancreas, brain and breast carcinoma tissues.
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常规说明
ab271907 is the carrier-free version of ab134152.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
UMB1 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab134152)
- Alexa Fluor® 488 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab311039)
- Alexa Fluor® 594 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab311816)
- Alexa Fluor® 568 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab313099)
- Alexa Fluor® 555 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab313292)
- Alexa Fluor® 750 Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (ab321081)
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab271907于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.Can be blocked with Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - C-terminal (ab134152).
This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
|
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| IP |
Use at an assay dependent concentration.
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.Can be blocked with Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - C-terminal (ab134152). This antibody works better in 1%SDS Hot Lysates in WB. For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
|
|
IP
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Receptor for somatostatins-14 and -28. This receptor is coupled via pertussis toxin sensitive G proteins to inhibition of adenylyl cyclase. In addition it stimulates phosphotyrosine phosphatase and PLC via pertussis toxin insensitive as well as sensitive G proteins. In RIN-5F cells, this receptor inhibits calcium entry by suppressing voltage dependent calcium-channels. -
组织特异性
Cerebrum and kidney. In lesser amounts in jejunum, colon and liver. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6752 Human
- Entrez Gene: 20606 Mouse
- Entrez Gene: 54305 Rat
- Omim: 182452 Human
- SwissProt: P30874 Human
- SwissProt: P30875 Mouse
- SwissProt: P30680 Rat
- Unigene: 514451 Human
see all -
别名
- Somatostatin receptor type 2 antibody
- SRIF-1 antibody
- SRIF1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)
Immunohistochemical analysis of formalin-fixed paraffin-embedded human pancreas labelling somatostatin receptor 2 with ab134152 at a concentration of 0.2µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab134152 anti somatostatin receptor 2 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)](https://www.abcam.cn/ps/products/271/ab271907/Images/ab271907-512446-anti-somatostatin-receptor-2-antibody-umb1-c-terminal-tma-map.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).
Tissue Microarrays stained for Anti-Somatostatin Receptor 2 antibody [UMB1] using ab134152 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negaive (cross mark) staining per sample type tested.The sections were incubated with ab134152 for 30 mins at room temperature used at 1:2000 dilution (0.928 μg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody. Counterstain was Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)](https://www.abcam.cn/ps/products/271/ab271907/Images/ab271907-511370-anti-somatostatin-receptor-2-antibody-umb1-c-terminal-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling Somatostatin Receptor 2 with ab134152 at 1/2000 dilution (0.928 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at a ready to use dilution. Positive staining on human breast carcinoma. The section was incubated with ab134152 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at a ready to use dilution.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)](https://www.abcam.cn/ps/products/271/ab271907/Images/ab271907-1-ab134152148421antisomatostatinreceptor2antibodyumb1cterminalimmunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)Immunohistochemical analysis of paraffin embedded human brain tissue labeled with ab134152 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)](https://www.abcam.cn/ps/products/271/ab271907/Images/ab271907-2-ab134152148422antisomatostatinreceptor2antibodyumb1cterminalimmunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)Immunohistochemical analysis of paraffin embedded human pancreas tissue labeled with ab134152 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152). -
![Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)](https://www.abcam.cn/ps/products/271/ab271907/Images/ab271907-4-benefits-of-recombinant-antibodies.png)
Anti-Somatostatin Receptor 2 antibody [UMB1] - BSA and Azide free (ab271907)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab271907 尚未被引用在任何文献中。
