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Anti-Sodium Potassium ATPase抗体- Plasma膜Marker (ab58475)

Reviews (1)Q&A (3)References (24)
Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)

Key features and details

  • Rabbit polyclonal to Sodium Potassium ATPase - Plasma Membrane Marker
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Sodium Potassium ATPase抗体- Plasma膜Marker
    参阅全部 Sodium Potassium ATPase 一抗

  • 描述

    兔多克隆抗体to Sodium Potassium ATPase - Plasma膜Marker

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic non-phosphopeptide derived from human Sodium Potassium ATPase around the phosphorylation site of serine 16 (A-V-SP-E-H).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab58475于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/50 - 1/100.
WB
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).
说明
IHC-P
1/50 - 1/100.
WB
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).

靶标

  • 功能

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

  • 序列相似性

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

  • 翻译后修饰

    Phosphorylation on Tyr-10 modulates pumping activity.

  • 细胞定位

    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • ATPase Na+/K+ transporting alpha antibody
    • adenosinetriphosphatase antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • ATP1A4 antibody
    • ATP1AL2 antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
    • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
    • Beta 1-subunit of Na(+),K(+)-ATPase antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na(+)/K(+) ATPase alpha-4 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na+/K+ ATPase 4 antibody
    • Na+/K+ ATPase, alpha-D polypeptide antibody
    • Na, K-ATPase beta-1 polypeptide antibody
    • Na, K-ATPase, alpha-A catalytic polypeptide antibody
    • Na,K-ATPase catalytic subunit alpha-A protein antibody
    • Na,K-ATPase subunit alpha-C antibody
    • polypeptide-like 2 antibody
    • Sodium pump 1 antibody
    • sodium pump 4 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium pump subunit alpha-4 antibody
    • sodium pump subunit beta-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-4 antibody
    • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
    • sodium-potassium ATPase, alpha 4 polypeptide antibody
    • sodium-potassium-ATPase, alpha 1 polypeptide antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase alpha 1 chain antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • sodium/potassium-dependent ATPase beta-1 subunit antibody
    • sodium/potassium-transporting ATPase alpha-4 chain antibody
    • sodium/potassium-transporting ATPase beta-1 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    • sodium/potassium-transporting ATPase subunit alpha-4 antibody
    see all

图片

  • Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
    Western blot - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
    All lanes : Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475) at 1/500 dilution

    Lane 1 : 293 cell extracts treated with PMA (125ng/ml, 30mins)
    Lane 2 : 293 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide

    Predicted band size: 113 kDa
    Observed band size: 113 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475)
    ab58475 at 1/50 dilution staining Sodium Potassium ATPase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

文献 (24)

发表研究结果有使用 ab58475？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab58475 被引用在 24 文献中.

  • Bhattacharya I  et al. Plasma Membrane-Derived Liposomes Exhibit Robust Antiviral Activity against HSV-1. Viruses 14:N/A (2022). PubMed: 35458528
  • Mandriani B  et al. Development of anti-somatostatin receptors CAR T cells for treatment of neuroendocrine tumors. J Immunother Cancer 10:N/A (2022). PubMed: 35764366
  • Kobuke K  et al. ATP1A1 Mutant in Aldosterone-Producing Adenoma Leads to Cell Proliferation. Int J Mol Sci 22:N/A (2021). PubMed: 34681640
  • Li L & Zhang C Venlafaxine Attenuated the Cognitive and Memory Deficit in Mice Exposed to Isoflurane Alone. Front Neurol 12:591223 (2021). PubMed: 33708168
  • Keshri GK  et al. Effects of Pulsed 810?nm Al-Ga-As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight. Lasers Surg Med 52:424-436 (2020). PubMed: 31483061
View all Publications for this product

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1-4 of 4 Abreviews or Q&A

Flow Cytometry abreview for Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker

 Excellent
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (trialed on kidney and spleen cells)
Permeabilization
No
Gating Strategy
renal tubule cells as gated by size and live B220+CD19+ cells (B cells)
Specification
trialed on kidney and spleen cells
Preparation
Cell harvesting/tissue preparation method: single cells suspension. Spleens were RBC lysed. Kidneys were digested with collagenase D and a Ficoll gradient was applied for isolation of lymphocyte populations
Sample buffer: PBS + 2% FCS + 1mM EDTA
Fixation
not fixed
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Irene Chernova

Verified customer

提交于 Mar 07 2019

Question

What is the concentration of the Optiblot DTT Reducer (10x) ab119199

Read More
Answer

The stock solution of the Optiblot DTT Reducer (10X) ab119199 in 2M. This means that once diluted 10 times this produces a 200 mM solution. Therefore if you usually use 10 mM with your samples I would suggest diluting this 200 times. The higher concentration should not really have an effect of the running of protein gels but as the pKa is around 8, in high concentration it can alter the pH somewhat which may affect the migration. We would normally suggest using 50 mM to ensure fully reducing the disulphide bonds in your samples.

Read More

Question

This product did not perform well in adipose tissue, can you suggest a cell membrane marker that may work?

Read More
Answer

Thank you for contacting us.   I have looked through our catalog of products searching for membrane marker that may perform better for you in adipose tissue. I concur with your assessment that an Anti-pan Cadherin antibody may be a better choice and would recommend ab6529. Other markers that may interest you are ab115978 Anti-EMA and ab58285 Anti-Paralemmin. I have included the links to each of these products below. https://www.abcam.com/pan-Cadherin-antibody-Plasma-Membrane-Marker-ab6529.html https://www.abcam.com/EMA-antibody-EMA-201-ab115978.html https://www.abcam.com/Paralemmin-antibody-ab58285.html I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

Read More

Question

I am interestend in purchasing the item sodium potassium ATPase antibody (ab58475) but if possible i would like to ask you some questions first: 1.Is this antibody specific for Na K ATPase alpha 1 subunit? 2.Does it react with Na K ATPase from monkey cells (Vero E6)? or it has not been tested yet Thank you very much in advance

Read More
Answer

Thank you for your enquiry. This antibody will detect the alpha 1 subunit and not the beta 1 subunit. As far as we are aware, cross reactivity with monkey has not yet been tested for use with ab58475. All species cross-reactivity information is stated on Abcam product datasheets, and these are updated as soon as any new information is brought to our attention. If you decide to go ahead and purchase this product, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, and how you can earn points for discounts on future purchases, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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