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Tags & Cell Markers Subcellular Markers Plasma Membrane

Anti-Sodium Potassium ATPase抗体(ab353)

  • Datasheet
  • SDS
Submit a review Q&A (4)References (10)

Product price, shipping and contact information

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Key features and details

  • Chicken polyclonal to Sodium Potassium ATPase
  • Suitable for: ELISA, IHC-Fr, ICC/IF, WB
  • Reacts with: Dog
  • Isotype: IgY

选择批间可重复性更高的重组抗体

Product image
Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Sodium Potassium ATPase抗体
    参阅全部 Sodium Potassium ATPase 一抗
  • 描述

    鸡多克隆抗体to Sodium Potassium ATPase
  • 宿主

    Chicken
  • 特异性

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney].
  • 经测试应用

    适用于: ELISA, IHC-Fr, ICC/IF, WBmore details
  • 种属反应性

    与反应: Dog
  • 免疫原

    Full length protein corresponding to Sodium Potassium ATPase. ATPase, Sodium, Potassium [Canine Kidney]

  • 阳性对照

    • ICC: MDCK cells ELISA: 1ug recombinant protein
  • 常规说明

    Centrifuge product if not completely clear after standing at room temperature. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please note that we have 2 concentrations of this antibody: 1mg/ml and 10mg/ml. Please check the vial label.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 0.424% Potassium phosphate, 0.878% Sodium chloride
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
  • 克隆

    多克隆
  • 同种型

    IgY
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Plasma Membrane
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
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    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
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    • Types of disease
    • Cancer
    • Isotype/Loading Controls
    • Loading Controls
    • NaKATPase

相关产品

  • Isotype control

    • Chicken IgY - Isotype Control (ab50579)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab353于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
Use at an assay dependent concentration.

This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit)and ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature.

IHC-Fr
Use at an assay dependent concentration.

(see review)

ICC/IF
Use at an assay dependent concentration.

(see review). Use amplification.

WB
Use at an assay dependent concentration.
说明
ELISA
Use at an assay dependent concentration.

This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit)and ABTS (2,2'-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature.

IHC-Fr
Use at an assay dependent concentration.

(see review)

ICC/IF
Use at an assay dependent concentration.

(see review). Use amplification.

WB
Use at an assay dependent concentration.

靶标

  • 功能

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • 序列相似性

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • 翻译后修饰

    Phosphorylation on Tyr-10 modulates pumping activity.
  • 细胞定位

    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P05023 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 别名

    • ATPase Na+/K+ transporting alpha antibody
    • adenosinetriphosphatase antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • ATP1A4 antibody
    • ATP1AL2 antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
    • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
    • Beta 1-subunit of Na(+),K(+)-ATPase antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na(+)/K(+) ATPase alpha-4 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na+/K+ ATPase 4 antibody
    • Na+/K+ ATPase, alpha-D polypeptide antibody
    • Na, K-ATPase beta-1 polypeptide antibody
    • Na, K-ATPase, alpha-A catalytic polypeptide antibody
    • Na,K-ATPase catalytic subunit alpha-A protein antibody
    • Na,K-ATPase subunit alpha-C antibody
    • polypeptide-like 2 antibody
    • Sodium pump 1 antibody
    • sodium pump 4 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium pump subunit alpha-4 antibody
    • sodium pump subunit beta-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-4 antibody
    • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
    • sodium-potassium ATPase, alpha 4 polypeptide antibody
    • sodium-potassium-ATPase, alpha 1 polypeptide antibody
    • Sodium/potassium dependent ATPase beta 1 subunit antibody
    • Sodium/potassium transporting ATPase alpha 1 chain antibody
    • Sodium/potassium transporting ATPase beta 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • sodium/potassium-dependent ATPase beta-1 subunit antibody
    • sodium/potassium-transporting ATPase alpha-4 chain antibody
    • sodium/potassium-transporting ATPase beta-1 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    • sodium/potassium-transporting ATPase subunit alpha-4 antibody
    see all

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (10)

发表研究结果有使用 ab353?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab353 被引用在 10 文献中.

  • Zámbó B  et al. Clinically relevant mutations in the ABCG2 transporter uncovered by genetic analysis linked to erythrocyte membrane protein expression. Sci Rep 8:7487 (2018). ICC/IF . PubMed: 29749379
  • Martín-Gago P  et al. Covalent Protein Labeling at Glutamic Acids. Cell Chem Biol 24:589-597.e5 (2017). PubMed: 28434875
  • Varga K  et al. Histone deacetylase inhibitor- and PMA-induced upregulation of PMCA4b enhances Ca2+ clearance from MCF-7 breast cancer cells. Cell Calcium 55:78-92 (2014). PubMed: 24439526
  • Braune EB  et al. S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development. Mol Cell Biol 34:1221-33 (2014). PubMed: 24449764
  • Liu R  et al. Itraconazole suppresses the growth of glioblastoma through induction of autophagy: involvement of abnormal cholesterol trafficking. Autophagy 10:1241-55 (2014). PubMed: 24905460
  • Antalffy G  et al. A C-terminal di-leucine motif controls plasma membrane expression of PMCA4b. Biochim Biophys Acta 1833:2561-72 (2013). ICC/IF . PubMed: 23830917
  • Czepiel M  et al. Differentiation of induced pluripotent stem cells into functional oligodendrocytes. Glia 59:882-92 (2011). PubMed: 21438010
  • Qin Y  et al. The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin. J Cell Biol 171:1061-71 (2005). ICC/IF ; Dog . PubMed: 16344308
  • Tauscher A & Kuver R ABCG5 and ABCG8 are expressed in gallbladder epithelial cells. Biochem Biophys Res Commun 307:1021-8 (2003). IF, IHC ; Dog . PubMed: 12878214
  • Lee J  et al. Polarized cholesterol and phospholipid efflux in cultured gall-bladder epithelial cells: evidence for an ABCA1-mediated pathway. Biochem J 364:475-84 (2002). PubMed: 12023891

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Question

ab35645: pas de marquage ICC/IF

Read More

Abcam community

Verified customer

Asked on Jun 11 2012

Answer

Merci de nous avoir contactés.

Après avoir effectué une recherche pour un anticorps marqueur de la membrane plasmique qui pourrait convenir à vos besoins (autre qu'un anticorps de lapin ou IgG1 de souris),je n'ai trouvé que l'anti-Sodium Potassium ATPase référence https://www.abcam.com/ab353 dont nous avons brièvement parlé au téléphone la semaine dernière.

Cet anticorps polyclonal de poulet (IgY)n'a pour l'instant été testé que chez le chien. Cependant, l'alignement de séquences entre les Sodium/potassium-transporting ATPase subunit alpha-1 humaine et canine montre une homologie de 97% (voirfichier pdf ci-joint). Ceci suppose que l'anticorps ab353 pourrait fonctionner chez l'humain.

Désirez-vous tester cet anticorps en remplacement du ab35645 qui n'a pas fonctionné et pour lequel nous n'avons malheureusement pas d'autre lot que celui que vous avez reçu?

Dans l'attente de votre réponse je vous souhaite une très bonne journée.

Read More

Abcam Scientific Support

回复于 Jun 11 2012

Question

I am interesting in buying an antibody that is specific to Sodium potassium ATPase, that is specific to a peptide of this protein that lies within the cytoplasm. I believe that most commercial ATPase antibodies are specific to regions that are exposed on the cell surface. Do you know whether one of your antibodies actually target the internal face of the plasma membrane?

Read More

Abcam community

Verified customer

Asked on Jan 23 2006

Answer

Thank you for your enquiry. The epitope has not been mapped in the cases of ab7671 and ab8344 therefore we do not know which part of the protein is recognised by those antibodies. As the immunogen used to generate ab353 is purified Na,K-ATPase from rabbit renal outermedulla and the antibody is a polyclonal antibody the product will recognise a number of epitopes on the protein, some are likely to be at the plasma membrane portion intracellularly and some extracellularly. I hope this information helps, please do not hesitate to contact us again if you need more information,

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Abcam Scientific Support

回复于 Jan 24 2006

Question

The Na pump has 2 subunits, alpha and beta. Which of them is recognized by this ab? How many bands do you see in western blot?

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Abcam community

Verified customer

Asked on Dec 18 2001

Answer

This antibody was found to be reactive against the intact enzyme and enzyme subunits (alpha at 100 kDa and beta at 50 kDa) as well as glycosylated and non-glycosylated enzyme forms by western blot. The product is also reactive against 1.0ug of ATPase, Sodium, Potassium in a standard sandwich ELISA.

Read More

Danielle Miller

Abcam Scientific Support

回复于 Dec 19 2001

Question

Does the ab to canine Na-K-ATPase crossreact with the human or rodent enzyme?

Read More

Abcam community

Verified customer

Asked on Jul 03 2001

Answer

Reactivity with other mammalian sources is unknown, but likely, due to the nature of the polyclonal antibody.

Read More

Neil Hayward

Abcam Scientific Support

回复于 Jul 09 2001

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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