Anti-SODD抗体(ab2048)
Key features and details
- Rabbit polyclonal to SODD
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SODD抗体
参阅全部 SODD 一抗 -
描述
兔多克隆抗体to SODD -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human SODD aa 2-16.
Sequence:SALRRSGYGPSDGPSC
(Peptide available asab8382) -
阳性对照
- HeLa whole cell lysate or THP-1 whole cell lysate.
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常规说明
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identified and designated SODD for SODDs. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
DEAE-Chromatography -
纯化说明
SODD Antibody is Antibody is DEAE purified. -
Primary antibody说明
Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several novel death receptors including DR3, DR4, DR5, and DR6 were recently identified. Cell death signal is transduced by death domain containing adapter molecules through the interaction with death domain of these death receptors. A novel TNF-R1 interacting protein was recently identifiedand designated SODD for silencer of death domains. SODD associates with the death domain of TNF-R1 and prevents constitutive activation of TNF-R1 signaling. TNF treatment releases SODD and permits adapter molecules such as TRADD recruiting to the active TNF-R1 complex, which activates TNF signaling pathways. SODD also interacts with DR3. SODD is ubiquitously expressed in human tissues and cell lines. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB |
1/500 - 1/2000. Detects a band of approximately 60 kDa.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
WB
1/500 - 1/2000. Detects a band of approximately 60 kDa. |
靶标
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功能
Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release (By similarity). Prevents constitutive TNFRSF1A signaling. -
组织特异性
Ubiquitous. -
序列相似性
Contains 1 BAG domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 9530 Human
- Omim: 603884 Human
- SwissProt: O95429 Human
- Unigene: 194726 Human
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别名
- BAG 4 antibody
- BAG family molecular chaperone regulator 4 antibody
- BAG-4 antibody
see all
图片
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Western blot - Anti-SODD antibody (ab2048)All lanes : Anti-SODD antibody (ab2048) at 1/500 dilution
Lane 1 : HeLa whole cell lysate with absence of blocking peptide
Lane 2 : THP-1 whole cell lysate with absence of blocking peptide
Lane 3 : HeLa whole cell lysate with SODD peptide (ab8382)
Lane 4 : THP-1 whole cell lysate with SODD peptide (ab8382)
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)ab2048 at 5µg/ml staining SODD in Hela cells by ICC/IF
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Immunocytochemistry/ Immunofluorescence - Anti-SODD antibody (ab2048)Immunofluorescence of SODD in Hela cells using ab2048 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab2048 被引用在 2 文献中.
- Yi L et al. Bcl-2 associated athanogene 4 promotes proliferation, migration and invasion of gastric cancer cells. Mol Med Rep 16:3753-3760 (2017). PubMed: 29067445
- Boiani M et al. The stress protein BAG3 stabilizes Mcl-1 protein and promotes survival of cancer cells and resistance to antagonist ABT-737. J Biol Chem 288:6980-90 (2013). WB ; Human . PubMed: 23341456