Anti-SOD2/MnSOD抗体(ab118340)
Key features and details
- Goat polyclonal to SOD2/MnSOD
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SOD2/MnSOD抗体
参阅全部 SOD2/MnSOD 一抗 -
描述
山羊多克隆抗体to SOD2/MnSOD -
宿主
Goat -
特异性
ab118340 is expected to recognize both reported isoforms (NP_000627.2; NP_001019637.1). The immunizing peptide represents the acetylation site including K122 according to isoform A. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Pig
预测可用于: Cow, Dog, Zebrafish
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免疫原
Synthetic peptide corresponding to Human SOD2/MnSOD aa 100-200 (internal sequence) (Cysteine residue).
Database link: P04179 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab118340 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118340于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa).
A 1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa). A 1 hour primary incubation is recommended for this product. |
靶标
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功能
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. -
疾病相关
Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
序列相似性
Belongs to the iron/manganese superoxide dismutase family. -
翻译后修饰
Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 6648 Human
- Entrez Gene: 20656 Mouse
- Entrez Gene: 24787 Rat
- Entrez Gene: 335799 Zebrafish
- Omim: 147460 Human
- SwissProt: P04179 Human
- SwissProt: P09671 Mouse
- SwissProt: P07895 Rat
see all -
别名
- Indophenoloxidase B antibody
- IPO B antibody
- IPOB antibody
see all
图片
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Western blot - Anti-SOD2/MnSOD antibody (ab118340)All lanes : Anti-SOD2/MnSOD antibody (ab118340)
Lane 1 : Human Cerebellum lysate
Lane 2 : Mouse Brain lysate
Lane 3 : Rat Brain lysate
Lane 4 : Pig Brain lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 25 kDa -
Western blot - Anti-SOD2/MnSOD antibody (ab118340)All lanes : Anti-SOD2/MnSOD antibody (ab118340)
Lane 1 : HeLa cell lysate
Lane 2 : HepG2
Lane 3 : HEK293
Lane 4 : NIH3T3
Lysates/proteins at 35 µg per lane.
Predicted band size: 25 kDa -
Western blot - Anti-SOD2/MnSOD antibody (ab118340)Anti-SOD2/MnSOD antibody (ab118340) at 0.1 µg/ml (incubation time: 1 hour) + Mouse Spinal Cord lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab118340 被引用在 2 文献中.
- Cai X et al. lncRNA FGD5 antisense RNA 1 upregulates RORA to suppress hypoxic injury of human cardiomyocyte cells by inhibiting oxidative stress and apoptosis via miR-195. Mol Med Rep 22:4579-4588 (2020). PubMed: 33174051
- Huang Y et al. Biological effects of pyrroloquinoline quinone on liver damage in Bmi-1 knockout mice. Exp Ther Med 10:451-458 (2015). PubMed: 26622336