Anti-SOD2/MnSOD 抗体

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (AB13534)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SOD2/MnSOD with ab13534. Cells were fixed with 2% formaldehyde for 20 minutes at room temperature and incubated with the primary antibody at a dilution of 1/120 for 12 hours at 4°C. Followed by incubation with a R-PE-conjugated goat anti-rabbit IgG at a dilution of 1/200 for 2 hours at room temperature. Counterstained with DAPI (blue) at 1/40000 for 2 hours at room temperature. Magnification : 100x.

Left : DAPI (blue) nuclear stain. Middle : SOD2/MnSOD. Right : Merge.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (AB13534)

ab13534 at 1/100 dilution staining Superoxide Dismutase 2 in mouse back skin tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin-embedded tissue section). Antigen retrieval was done by microwave in citrate buffer. A Fluorophore-conjugated goat anti-rabbit secondary was used at 1/50 dilution.

• ICC/IF

AbReview10512****

Immunocytochemistry/ Immunofluorescence - Anti-SOD2/MnSOD antibody (AB13534)

ab13534 staining differentiating (rat) PC12 cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.2% Triton X100 prior to blocking in 10% serum at RT for 1 hour. The primary antibody was incubated with the sample for 1 hour at 2 μg/ml. An Alexa Fluor^® 488 conjugated goat anti-rabbit antibody diluted 1/1000 was used as the secondary. Cells were counterstained with the nuclear TOPRO-3 stain.

This image is courtesy of an Abreview submitted by Dr Anne Chiaramello

• IHC-P

AbReview35090****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOD2/MnSOD antibody (AB13534)

ab13534 staining SOD2 in Human colon and Horse Ileo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/2000) for 18 hours.

This image is courtesy of an anonymous Abreview

• WB

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Western blot - Anti-SOD2/MnSOD antibody (AB13534)

All lanes:

Western blot - Anti-SOD2/MnSOD antibody (ab13534) at 1/1000 dilution

All lanes:

Rat Tissue lysates at 15 µg

Secondary

All lanes:

Donkey Anti-Rabbit IgG: HRP

false