Anti-SOCS7抗体(ab224589)
Key features and details
- Rabbit polyclonal to SOCS7
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-SOCS7抗体
参阅全部 SOCS7 一抗 -
描述
兔多克隆抗体to SOCS7 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Recombinant fragment corresponding to Human SOCS7 aa 6-165.
Database link: O14512 -
阳性对照
- WB: MCF7, HeLa, Jurkat and U-87 MG cell lysate; rat heart and liver tissue lysate. ICC/IF: HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224589于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/200.
|
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| WB |
1/500 - 1/5000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
|
| 说明 |
|---|
|
ICC/IF
1/50 - 1/200. |
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WB
1/500 - 1/5000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
靶标
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功能
Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
组织特异性
Expressed in brain and leukocytes. Also in fetal lung fibroblasts and fetal brain. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
结构域
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes (By similarity). It is required for IRS1 ubiquitination and subsequent proteasomal degradation. -
细胞定位
Cytoplasm. Cell membrane. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Rapidly relocalizes to the nucleus after UV irradiation. Cytoplasmic location depends upon SEPT7 presence. - Information by UniProt
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数据库链接
- Entrez Gene: 30837 Human
- Entrez Gene: 287659 Rat
- Omim: 608788 Human
- SwissProt: O14512 Human
- Unigene: 514132 Human
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别名
- Ash and phospholipase C gamma-binding protein antibody
- NAP 4 antibody
- NAP 4 Fragment antibody
see all
图片
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Western blot - Anti-SOCS7 antibody (ab224589)All lanes : Anti-SOCS7 antibody (ab224589) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 5 : Rat heart tissue lysate
Lane 6 : Rat liver tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-SOCS7 antibody (ab224589)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SOCS7 (green) using ab224589 at 1/100 dilution in ICC/IF.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab224589 被引用在 4 文献中.
- Du Y et al. SOCS7/HuR/FOXM1 signaling axis inhibited high-grade serous ovarian carcinoma progression. J Exp Clin Cancer Res 41:185 (2022). PubMed: 35624501
- Wang C et al. Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation. Front Oncol 11:668743 (2021). PubMed: 33968776
- Ding R et al. DZNep, an inhibitor of the histone methyltransferase EZH2, suppresses hepatic fibrosis through regulating miR-199a-5p/SOCS7 pathway. PeerJ 9:e11374 (2021). PubMed: 34040893
- Su M et al. miR-885-5p upregulation promotes colorectal cancer cell proliferation and migration by targeting suppressor of cytokine signaling. Oncol Lett 16:65-72 (2018). WB ; Human . PubMed: 29928388