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Immunology Innate Immunity Cytokines IL6 Related

Anti-SOCS3抗体(ab723)

  • Datasheet
Reviews (1)Q&A (3)References (1)

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Western blot - Anti-SOCS3 antibody (ab723)

    Key features and details

    • Goat polyclonal to SOCS3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-SOCS3抗体
      参阅全部 SOCS3 一抗
    • 描述

      山羊多克隆抗体to SOCS3
    • 宿主

      Goat
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Rat
    • 免疫原

      Synthetic peptide: PIREFLDQYDAPL, corresponding to C terminal amino acids 213-225 of Human SOCS3 .

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • MOLT-4 lysate
    • 常规说明

      Principal Names - SOCS3; CIS3; Cish3; SSI-3; SOCS-3; suppressor of cytokine signaling 3; STAT induced STAT inhibitor 3; cytokine-induced SH2 protein 3 Official Gene Symbol - SOCS3 GenBank Accession Number – NP_003946 LocusLink ID - 9021 (human); 12702 (mouse); 89829 (rat) Gene Ontology terms - protein kinase inhibitor; JAK-STAT cascade; anti-apoptosis Size - 225 aa

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.05% Tris, 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Immunology
      • Innate Immunity
      • Cytokines
      • IL6 Related
      • Signal Transduction
      • Adapters
      • Cytoplasmic
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • STATs
      • Stem Cells
      • Hematopoietic Progenitors
      • Intracellular Molecules
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Obesity
      • Metabolism
      • Types of disease
      • Cancer

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Positive Controls

      • MOLT-4 whole cell lysate (ab7912)
    • Recombinant Protein

      • Recombinant Human SOCS3 protein (ab131811)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab723于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    Use a concentration of 1 - 3 µg/ml.
    Approx 24kDa band seen in MOLT-4 lysate. Recommended for use at 4.0-6.0µg/ml. Block membrane in 2.5% skimmed milk in TBST (TBS + 0.05% Tween). Incubate primary antibody for 1 hour at room temperature with agitation.
    说明
    WB
    Use a concentration of 1 - 3 µg/ml.
    Approx 24kDa band seen in MOLT-4 lysate. Recommended for use at 4.0-6.0µg/ml. Block membrane in 2.5% skimmed milk in TBST (TBS + 0.05% Tween). Incubate primary antibody for 1 hour at room temperature with agitation.

    靶标

    • 功能

      SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
    • 组织特异性

      Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
    • 通路

      Protein modification; protein ubiquitination.
    • 疾病相关

      Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
    • 序列相似性

      Contains 1 SH2 domain.
      Contains 1 SOCS box domain.
    • 结构域

      The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
      The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
    • 翻译后修饰

      Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
    • Target information above from: UniProt accession O14543 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 9021 Human
      • Entrez Gene: 12702 Mouse
      • Entrez Gene: 89829 Rat
      • Omim: 604176 Human
      • SwissProt: O14543 Human
      • SwissProt: O35718 Mouse
      • SwissProt: O88583 Rat
      • Unigene: 527973 Human
      • Unigene: 3468 Mouse
      • Unigene: 127801 Rat
      see all
    • 别名

      • ATOD4 antibody
      • CIS 3 antibody
      • CIS-3 antibody
      • CIS3 antibody
      • Cish3 antibody
      • Cytokine induced SH2 protein 3 antibody
      • Cytokine-inducible SH2 protein 3 antibody
      • E2a Pbx1 target gene in fibroblasts 10 antibody
      • EF 10 antibody
      • MGC71791 antibody
      • SOCS 3 antibody
      • SOCS-3 antibody
      • Socs3 antibody
      • SOCS3_HUMAN antibody
      • SSI 3 antibody
      • SSI-3 antibody
      • SSI3 antibody
      • STAT induced STAT inhibitor 3 antibody
      • STAT-induced STAT inhibitor 3 antibody
      • Suppressor of cytokine signaling 3 antibody
      see all

    图片

    • Western blot - Anti-SOCS3 antibody (ab723)
      Western blot - Anti-SOCS3 antibody (ab723)
      Anti-SOCS3 antibody (ab723) at 1 µg/ml + MOLT-4 lysate at 35 µg

      Observed band size: 24 kDa why is the actual band size different from the predicted?



      Detected by western blot using chemiluminescence.

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab723?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab723 被引用在 1 文献中.

    • Minter MR  et al. Deletion of the type-1 interferon receptor in APPSWE/PS1?E9 mice preserves cognitive function and alters glial phenotype. Acta Neuropathol Commun 4:72 (2016). WB . PubMed: 27400725

    客户评价及客户问答

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-SOCS3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Muscle)
    Gel Running Conditions
    Reduced Denaturing (3)
    Loading amount
    45 µg
    Specification
    Muscle
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Dec 21 2017

    Question

    We have decided to order the antibody SOCS 3 ( cat.no. ab723 ) and hopefully we have a good staining on the rat sections. Can you tell me the price in euro's ( + taxes )? Is there something known about the deliverytime?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 24 2004

    Answer

    Thank you for your email. Ab723 is 325 Euros and is in stock in our office in Great Britain so upon ordering, you should receive it within a couple of days. You can place your order online, or via fax, email or phone. Below is the appropriate contact details: Abcam Ltd 21 Cambridge Science Park Milton Road Cambridge CB4 0TP UK Email: orders@abcam.com Phone: +44 1223 472030 Fax: +44 1223 472038 Office hours: 08.30 - 17.00 hrs (UK) Monday to Friday Please let us know how ab723 works out for you and contact me again if you have any more questions.

    Read More

    Abcam Scientific Support

    回复于 Aug 24 2004

    Question

    We are interested in the SOCS 3 antibody ( cat.no. ab 723 ). I have some questions: Does this antibody work on rat material? We are using fresh or paraformaldehyde fixed rat brain tissue. The sections are vibratome sections and the technique is IHC.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 10 2004

    Answer

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with rat has not yet been tested for use with ab723. The antibody cross-reacts with human and is expected to cross-react with rat and mouse due to sequence homology. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. Please let me know if you require any further assistance.

    Read More

    Abcam Scientific Support

    回复于 Aug 10 2004

    Question

    Here is the answer to your questions. 1.High backgroung, several bands trouhgout the blot. The positive control did not show in the blot ( molt 4 whole cell lysate) 2.Human left ventricle, total protein 3.45ug of total protein. I used a 2% SDS lysis buffer with protein I nhibitors. I boiled the sample for ~10 min at 100 degree Celsius. 4.It was raided in goat ( AB723). I use different dilutions ranging from 2ug/ml to 6ug/ml. I incubated it for 1 hr at room temperature, and also overnight at 4C 5.Secondary antibody was raised in rabbit. I tested it from 1:2000 to 1:10000. I incubate the secondary antibody for one hour at room temp. I wash a total of 4X 35 -40min. I tested my secondary antibody and I got no background with it. 6.ECL 7.Yes I run a positive control that I bought from you ( AB7912) I got no band. I did tested the secondary antibody, and I got no background. 8.I performed the western 4 times. Yes I get the background bands in the same place. I change concentrations for the 1AB and 2AB. I also have changed the incubation times (overnight).

    Read More

    Abcam community

    Verified customer

    Asked on Apr 23 2004

    Answer

    Thank you for the details that you have provided. We have had no other complaints concerning this antibody. The antibody's originator has provided me with the following protocol that they used when testing the antibody which should be helpful for you: - Lysis. Cell pellets were washed with ice-cold PBS. 1 ml of RIPA buffer was added per 1E8 cells and incubated on ice for 20 min, vortexing 2-3 times, briefly. The lysate was aliquotted into 1.5 ml microfuge tubes and centrifuged at 13,000 rpm for 5 min in a microfuge. The supernatant was transferred into clean tubes and its protein concentration was measured with BioRad protein assay. The concentration was then adjusted to 5 mg/ml with RIPA lysis buffer. An equal volume of 2 x SDS sample buffer was then added and the cell lysate was boiled for 5 minutes. Lysates were stored at -80C until use.(RIPA buffer = 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM PMSF, 1 mM EDTA, 5 µg/ml Aprotinin, 5 µg/ml Leupeptin, 1% Triton -100, 1% Sodium deoxycholate, 0.1% SDS). - SDS PAGE. Samples were run at 200V constant on a 12% acrylamide SDS-PAGE mini gel - using Biorad Mini-Protean 3 kit and protocols. Before loading samples had 5% (v/v) 2-ME added and were boiled for 3 minutes. - Transfer. We used a Biorad Mini Trans-Blot, constant 100 V for 1 hour. Transfer Buffer was 20 mM Tris pH 8.0, 150 mM Glycine, 10% Methanol. We transferred to Millipore PVDF membrane and stained with Ponceau Red to evaluate the transfer. - Staining. The membrane was blocked in 2.5% skimmed milk in TBS-T (TBS + 0.05% Tween-20) for 1 hr at room temperature with agitation. Primary antibody was incubated for 1 hr at room temperature with agitation. We used sigma secondary (Sigma anti-goat-HRP Product # A4174, use 1:3000) for 1 hr at room temperature with agitation. We washed with TBST three times after primary and secondary antibody, each wash lasting for 5-10 mins. ECL-plus (Amersham) was used rather than ECL, which is considerably more sensitive. Final detection was on autoradiography film. As you got no signal with the positive control, MOLT-4 lysate, I am willing to give you a replacement batch of this antibody if you would like. Please let me know what you would like to do.

    Read More

    Abcam Scientific Support

    回复于 Apr 27 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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